一种入侵我国的新害虫——桧柏臀纹粉蚧（半翅目：蚧次目：粉蚧科）
A new invasive pest in China: Planococcus vovae (Nassonov, 1909) (Hemiptera: Coccomorpha： Pseudococcidae)
武三安，李宇昂，徐 晗
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DOI：10.7679/j.issn.2095-1353.2023.089
作者单位：北京林业大学森林培育与保护省部共建重点实验室，北京 100083
中文关键词：桧柏臀纹粉蚧；杜松臀纹粉蚧；入侵害虫；北京
英文关键词：Planococcus vovae; P. juniperus; invasive pest; Beijing
中文摘要：
【目的】 确定杜松臀纹粉蚧是否为桧柏臀纹粉蚧Planococcus vovae异名，进而探明桧柏臀纹粉蚧是否为我国新入侵害虫。【方法】 除比较雌成虫形态外，基于COI的HCO-LCO区域序列，利用NCBI的基因数据库的BLAST功能对我国北京和内蒙古粉蚧样品进行分子鉴定；结合7种粉蚧，构建贝叶斯和最大似然系统发育树；计算样品间的遗传距离。【结果】 内蒙古和北京样品的雌成虫间存有明显的形态差别；北京和内蒙古样品与法国桧柏臀纹粉蚧的COⅠ匹配度分别为99.85%和95.13%；在贝叶斯和最大似然系统发育树上，北京样品与法国和西班牙的桧柏臀纹粉蚧聚为1支，内蒙古样品则与其为姐妹群；北京3份样品间及与法国、西班牙的桧柏臀纹粉蚧间遗传距离为0，内蒙古与北京样品间的遗传距离为0.05。【结论】 杜松臀纹粉蚧不是桧柏臀纹粉蚧异名，应恢复其种级地位；确定桧柏臀纹粉蚧是我国一种新入侵害虫。
英文摘要：
[Objectives] To determine whether Planococcus vovae (Nassonov, 1909) is synonymous with Planococcus juniperus Tang, 1989 or a new invasive pest in China. [Methods] In addition to comparing the morphology of adult females collected from Beijing and Inner Mongolia, new sequences in the HCO-LCO region of the COI gene were compared to those in NCBI using the Basic Local Alignment Search Tool (BLAST. Phylogenetic trees were then constructed using the Bayesian inference and Maximum likelihood methods, and the genetic distance between the COI sequences of different specimens was calculated. [Results] Many morphological differences are apparent between adult females from Beijing and those from Inner Mongolia. COI consistency between specimens from Beijing and Inner Mongolia, and between specimens from Beijing and French P. vovae, are 99.85% and 95.13%, respectively. Beijing specimens and P. vovae from France and Spain clustered in a single clade on two phylogenetic trees and had a genetic distance of 0.In contrast, there is a genetic distance of 0.05 between European specimens and those from from Inner Mongolia. [Conclusion] P. vovae is not synonymous with P. juniperus Tang and is therefore a new invasive pest in China.