摘  要  【目的】 本研究旨在明确热激蛋白P-Hsp70基因表达对不同温度下方斑瓢虫Propylaea quatuordecimpunctata成虫寿命和繁殖力的影响。【方法】 采用显微注射递送dsRNA的方法对方斑瓢虫成虫的P-Hsp70基因进行干涉，利用qRT-PCR检测方法计算P-Hsp70基因的干扰效率，同时观察P-Hsp70基因被干扰后方斑瓢虫成虫暴露在不同温度（32、35和38 ℃）下的存活和繁殖情况。【结果】 显微注射递送dsRNA的方法可有效抑制方斑瓢虫成虫P-Hsp70基因的表达。显微注射ds-P-Hsp70处理组方斑瓢虫成虫在各个温度下的存活率、寿命和繁殖力均显著低于未注射dsRNA的对照组。在32、35和38 ℃下，显微注射dsRNA 24 h后方斑瓢虫成虫存活率较对照组分别降低了15.5%、16.7%和56.8%，成虫平均寿命分别缩短40.6%、51.2%和65.8%。在32和35 ℃下，显微注射dsRNA成虫繁殖力分别降低了37.5%和100.0%；在38 ℃时，无论注射dsRNA与否，成虫均未能产卵。【结论】 热激蛋白P-Hsp70基因在方斑瓢虫成虫抵御高温胁迫过程中具有非常重要的作用。

Abstract  [Aim]  This study aims to investigate the effects of heat shock protein P-Hsp70 gene expression on the longevity and fecundity of adult 14-spotted ladybugs, Propylaea quatuordecimpunctata, under different temperatures. [Methods] Microinjection delivery of dsRNA was used to interfere with the P-Hsp70 gene of adult P. quatuordecimpunctata. qRT-PCR was used to calculate the interference efficiency of the P-Hsp70 gene. The adults were then exposed to different temperatures (32, 35, and 38 ℃) to determine the effect of temperature on their survival and reproduction following P-Hsp70 gene interference. [Results]  Our results demonstrate that microinjection delivery of dsRNA can effectively inhibit the expression of the P-Hsp70 gene in adult P. quatuordecimpunctata. The survival rate, longevity, and fecundity of adult P. quatuordecimpunctata treated with microinjection ds-P-Hsp70 were significantly lower than the control group (without injection of dsRNA) under all temperatures. At 32, 35, and 38 ℃, the survival rate of adults decreased by 15.5%, 16.7%, and 56.8%, respectively. Additionally, the average adult longevity was reduced by 40.6%, 51.2%, and 65.8%, respectively. At 32  and 35 ℃, the fecundity of adults decreased by 37.5% and 100.0%, respectively. At 38 ℃, the adults failed to lay eggs with or without dsRNA. [Conclusion]  Our findings indicate that the heat shock protein P-Hsp70 gene plays a crucial role in high temperature stress resistance in adult P. quatuordecimpunctata.