Wolbachia在北京地区小菜蛾种群中的感染
Infection of Wolbachia inPlutella xylostella in Beijing area of China
宋 月,王 哲,刘宏岳,沈佐锐**
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DOI：
作者单位：中国农业大学农学与生物技术学院 昆虫学系 北京 100094
中文关键词：
英文关键词：Wolbachia， Plutella xylostella， infection, Plutellidae
中文摘要：Wolbachia是一类广泛分布于节肢动物体内，可以引起其寄主生殖行为改变的细胞内共生细菌。小菜蛾
Plutella xylostella
(L.)是常见的鳞翅目害虫，严重危害十字花科植物。通过对
Wolbahcia的wsp基因的PCR扩增及克隆测序，明确
Wolbachia在北京地区小菜蛾野生种群内及其个体体内的分布情况。研究发现，在北京地区小菜蛾的野生种群中，感染了一种
Wolbahcia，将其命名为
wXyl，并在GenBank中进行注册；在小菜蛾成虫的个体体内，其胸部，腹部及足内都有
Wolbachia分布，而且所感染的
Wolbachia完全相同。
wXyl属于
Wolbachia B组中的
Pip亚组。研究首次发现在我国小菜蛾的野生种群内有
Wolbachia感染的现象。
英文摘要：Wolbachia is a common and widespread group of bacteria found in many arthropods. They are transmitted through the cytoplasm of eggs and have evolved various mechanisms for manipulating reproduction of their hosts. Plutella xylostella (L.) is a common lepidopteran pest of cruciferous vegetables. In this study, a strain of Wolbachia was detected in a natural population of P. xylostella in Beijing, based on PCR amplification of its wsp gene, cloning and sequencing. The sequenced wsp gene was named as wXyl. It was found that Wolbachia are distributed in the thorax, abdomen and legs of P. xylostella adults detected. wXyl belongs to the subgroup Pip in supergroup B. This is the first report that Wolbachia is distributed in a natural population of P. xylostella in China.