非嗜食寄主气味蛹期处理对不同寄主来源美洲斑潜蝇雌成虫嗅觉定向的影响
Influence of exposure to common host plant leaf volatile in pupal stage on the olfactory orientation of adults of Liriomyza sativae reared on different hosts
喻国辉1,2；程 萍1；古德祥2；张文庆2
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作者单位：1珠海市农业科学研究中心 珠海 519075；2中山大学昆虫学研究所 有害生物控制与资源利用国家重点实验室 广州 510275
中文关键词：美洲斑潜蝇， 寄主， 化学环境， 成虫定向行为
英文关键词：Liriomyza sativae, host, chemical environment, adult orientation behavior
中文摘要：测定用菜豆和丝瓜饲养的美洲斑潜蝇Liriomyza sativae Blanchard 蛹暴露于用正己烷稀释的番茄或南瓜叶片挥发性抽提物（TLE或PLE）72 h后，羽化雌成虫在Y形嗅觉仪中对TLE或PLE的定向率、平均反应时间以及对正己烷的定向率和平均反应时间，比较幼虫寄主经历和寄主气味对美洲斑潜蝇成虫嗅觉记忆的影响。结果表明，蛹期使用非嗜食寄主植物气味处理，能增加美洲斑潜蝇羽化雌成虫对非嗜食寄主气味的定向；幼虫期的寄主经历对处理后的成虫嗅觉定向也产生影响，饲喂丝瓜的美洲斑潜蝇蛹期处理后，羽化雌成虫显著偏向选择处理气味而非正己烷，饲喂菜豆的美洲斑潜蝇对南瓜的气味也表现出同样的特点，但对番茄的气味则相反，即使处理后对番茄气味的选择比率增加，仍然偏向选择正己烷。
英文摘要：Pupae of Liriomayza sativae Blanchard reared on the most favorite host kidney bean, and common host towel gourd, were exposed to tomato leaf volatile extraction (TLE) or pumpkin leaf volatile extraction (PLE) for 72 h, which were diluted by hexane. The orientation ratio (OR) and average responding time (AR) of females emerged from the treated pupae to the treatment compounds and hexane were investigated with Y type olfactometer. The results showed that the OR to the treatment compounds was enhanced after treated by the common host volatile compounds in pupa stage. The olfactory orientation of females was also influenced by the host experience in larva stage. For those females reared on towel gourd, after treated with PLE or TLE, the emerged females preferred to choose treatment compounds rather than hexane. The females reared on the most favorite host kidney bean; after treated with PLE, preferred to choose PLE, rather than hexane; but after treated with TLE, the females still preferred to choose normal hexane instead of TLE.