螨类系统学研究中的分子标记
The molecular markers in the systematic study of mites
张 旭1，2;金道超1**;郭建军1;朱 群1
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作者单位：1贵州大学昆虫学研究所 贵阳 550025；2淮北煤炭师范学院生物系 安徽 淮北 235000
中文关键词：螨类系统学, RAPD, DALP, AFLP, RFLP, SSR, 核酸序列分析
英文关键词：mite systematics, RAPD, DALP, AFLP, RFLP, SSR, DNA sequence analysis
中文摘要：近年来，分子标记技术在螨类系统学研究中起到越来越重要的作用。文章就几种螨类系统学中用到的分子标记的原理和应用作一回顾，其中包括随机扩增多态性RAPD、限制性内切酶片段长度多态性RFLP、直接扩增片段长度多态性DALP、扩增片段长度多态性AFLP、微卫星DNA SSR、核酸序列分析。讨论这几种分子标记在其应用中的优势及其局限性，并对分子标记在螨类系统学研究中的应用作出展望。
英文摘要：The application of molecular markers has become more and more important in the systematic study of mites recently. The principles and characteristics of several molecular markers, RAPD(Random Amplified Polymorphic), DALP(Direct Amplification of Length Polymorphism), AFLP(Amplified Fragment Length Polymorphism), RFLP(Restriction Fragment Length Polymorphism） SSR(Simple Sequence Repeat) and DNA Sequence Analysis which have been used in the systematic study of mites are described. The applications of these markers, in the context of mite systematic research, are reviewed. The advantages and disadvantages as their application were discussed in this paper. In addition, the applications prospects of molecular markers for the systematic study of mites were also presented.