. 室内模拟田间杀虫剂施药方式，研究3种杀虫剂连用及其顺序轮用对烟蚜Myzus persicae(Sulzer)抗药性发展和羧酸酯酶活力的影响。结果表明，氧化乐果、灭多威、高效氟氯氰菊酯连续施药9次后，其抗性分别增长73.3，8.9，10.4倍；氧化乐果→灭多威→高效氟氯氰菊酯顺序轮用3次（施药9次）后，氧化乐果抗性指数增长了47.3倍，灭多威抗性指数增长了6.7倍，高效氟氯氰菊酯抗性指数增长了5.0倍。羧酸酯酶活力检测结果表明，3种杀虫剂连用9次后，烟蚜种群的α-NA CarE活力分别增长了20.0，24.0和15.6倍，酶活力在0.6 (OD_600nm/aphid/min)以上的个体比例分别增加了73.4%，87.6%和43.8%；而3种杀虫剂顺序轮用3次（施药9次）后，烟蚜种群的αNA CarE活力增长了10.2倍，酶活力在0.6以上的个体比例增加了4.8%。结果证实杀虫剂轮换施用能延缓烟蚜抗药性的发展和烟蚜种群α-NA CarE活力及高活力个体频率的增加。
  

The effects of continuous use and rotational use of insecticides on the resistance developing and the carboxylesterase activity of Myzus persice(Sulzer) were studied by simulating used in field. The results showed that resistance of M. persice treated by nine continuous spraying to omethoate，meyhomyl and λcyhalothrin increased respectively by 54.2fold，8.9fold and 10.4fold，and the activity of α-NA carboxylesterase increased respectively by 20.0fold，24.0fold and 43.2fold，and the individuals with enzyme activity of 0.6 eased respectively by73%，87.6% and 43.8%. The resistance of M. persicae treated by three rotational spraying to omethoate，meyhomyl and λcyhalothrin increased respectively by 38.0fold，6.7fold and 5.0fold，and the enzyme activity increased by 10.2fold. The individuals with enzyme activity of 0.6 increased by 4.8% after treated by three rotations in order of omethoate，meyhomyl and λcyhalothrin. The resultsproved that rotational use of insecticides can delay the resistance developing，and decrease the carboxylesterase activity and the individuals with higher enzyme activity.