肥须亚麻蝇幼虫头咽骨形态学分析及其法医学意义
Chronometrical morphology changes of larval cephalopharyngeal skeleton in the different developmental stages of Parasarcophaga crassipalpis and its application in forensic medicine
王 玲1*；刘 彬2；李泽民1**
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作者单位：1河北医科大学病原生物学教研室 石家庄 050017；2河北省医学生物技术重点实验室 石家庄 050017
中文关键词：法医昆虫学，肥须亚麻蝇，幼虫头咽骨，死后间隔时间，温度
英文关键词：forensic entomology, Parasarcophaga crassipalpis, larval cephalopharyngeal skeleton, postmortem interval (PMI), temperature
中文摘要：为了明确肥须亚麻蝇Parasarcophaga crassipalpis Macguart幼虫头咽骨形态学特征的变化规律，分别在16，20，24，28和 32℃下饲养肥须亚麻蝇幼虫并定期取样, 显微镜下观察其形态变化，利用图像分析软件测量其口钩和咽骨的面积、平均光密度及骨化面积等形态参数。结果显示：在所测量口钩和咽骨的7项指标中，咽骨的平均光密度及骨化面积是最理想的幼虫日龄的判断指标，对尸体上死后间隔时间的推测具有重要的指导意义；口钩部的4项指标在龄期更叠时有明显变化， 仅可进行龄期的鉴别。
英文摘要： The important forensic fly, Parasarcophaga crassipalpis, Macquart was reared at different temperatures to examine its cephalopharyngeal skeleton morphological changes during growth. Some indexes including areas of different parts of cephalopharyngeal skeleton and average optical density had been measured through digital image processing technology. The results showed that the structural traits of pharyngeal sclerite regularly changed with daily larval growth and could be used as larval age markers, especially within the third instar. The indicators of mouth hook varied distinctively when instars overlapped. This study showed P. crassipalpis could be used as indicator species when establishing postmortem interval as long as the investigator accounted for the development data of the species.