一株白僵菌菌株的分离鉴定及对大猿叶虫的生物活性测定
Isolation and identification of a strain of Beauveria sp. and its biological activity to Colaphellus bowringi.
李红霞,于洪春**, 席贤举
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作者单位：东北农业大学农学院植物保护系 哈尔滨 150030
中文关键词：白僵菌,分离鉴定, 培养基， 大猿叶虫， 生物测定
英文关键词：Beauveria brongniartii, isolation and identification, culture medium, Colaphellus bowringi, biological activity test
中文摘要：
从田间土壤中自然感染死亡的大猿叶虫Colaphellus bowringi Baly僵尸上分离出一株白僵菌，经分离纯化培养鉴定为一株卵孢白僵菌Beauveria brongniartii。通过简易培养基的筛选试验，获得对该菌株具有较好培养效果和廉价的甜菜大豆培养基，并进行该菌株对大猿叶虫的活性测定。生物测定结果表明，该菌株对大猿叶虫幼虫感染率很低，但对其成虫具有很高的杀虫活性，以1×108孢子/mL的菌液处理土壤表面后15 d，成虫平均校正僵死率达81.7%，田间土壤处理后1个月，成虫校正死亡率达85.2%。表明该菌株适用于大猿叶虫的田间生物防治。
英文摘要： Through a series of cultivation and purification, a strain of Beauveria sp. was isolated from a dead body of Colaphellus bowringi Baly in field soil. It was identified to be Beauveria brongniartii. A better and cheaper culture medium was made up of sugar beet and soybean. The biological activity to C. bowringi was tested. The results showed that this strain can not effectively infect the larvae of C. bowringi, but there was higher insecticidal activity to adults. The soil was sprayed with a suspension of fungal spores with 1×108 mL-1 and the corrected mortality of adults was 81.7% after 15 days. The result of field experiment showed that this strain had better control effect, which reached adults mortality of 85.2% after a month. It indicated that this strain of B. brongniartii can be applied to biological control of C. bowringi in field.