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一株白僵菌菌株的分离鉴定及对大猿叶虫的生物活性测定
Isolation and identification of a strain of Beauveria sp. and its biological activity to Colaphellus bowringi.
李红霞,于洪春**, 席贤举
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DOI:
作者单位:东北农业大学农学院植物保护系 哈尔滨 150030
中文关键词:白僵菌,分离鉴定, 培养基, 大猿叶虫, 生物测定
英文关键词:Beauveria brongniartii, isolation and identification, culture medium, Colaphellus bowringi, biological activity test
中文摘要:


      从田间土壤中自然感染死亡的大猿叶虫Colaphellus bowringi Baly僵尸上分离出一株白僵菌,经分离纯化培养鉴定为一株卵孢白僵菌Beauveria brongniartii。通过简易培养基的筛选试验,获得对该菌株具有较好培养效果和廉价的甜菜大豆培养基,并进行该菌株对大猿叶虫的活性测定。生物测定结果表明,该菌株对大猿叶虫幼虫感染率很低,但对其成虫具有很高的杀虫活性,以1×108孢子/mL的菌液处理土壤表面后15 d,成虫平均校正僵死率达81.7%,田间土壤处理后1个月,成虫校正死亡率达85.2%。表明该菌株适用于大猿叶虫的田间生物防治。
英文摘要:

     Through a series of cultivation and purification, a strain of Beauveria sp. was isolated from a dead body of Colaphellus bowringi Baly in field soil. It was identified to be Beauveria brongniartii. A better and cheaper culture medium was made up of sugar beet and soybean. The biological activity to C. bowringi was tested. The results showed that this strain can not effectively infect the larvae of C. bowringi, but there was higher insecticidal activity to adults. The soil was sprayed with a suspension of fungal spores with 1×108 mL-1 and the corrected mortality of adults was 81.7% after 15 days. The result of field experiment showed that this strain had better control effect, which reached adults mortality of 85.2% after a month. It indicated that this strain of B. brongniartii can be applied to biological control of C. bowringi in field.

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