家蝇胚胎细胞系的建立及其生物学特性
Establishment of cell line of Musca domestica embryo
贺莉芳**,万启惠,刘 晖, 张 曦 阎玉涛
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DOI：
作者单位：遵义医学院寄生虫学教研室 遵义 563003
中文关键词：家蝇, 胚胎, 细胞系, 生物学特性
英文关键词：Musca domestica, embryo, cell line, biological characteristics
中文摘要：
昆虫细胞系在许多领域得到广泛的应用。以家蝇Musca domestica L.卵（胚胎）为材料
，用含20%胎牛血清的M3培养基培养，原代培养35 d左右形成单层，每7 d传代1次，连续传
代60代次。细胞群体倍增时间为44 h，染色体2n=12，可在液氮及4℃冰箱中保存复苏，命
名为MDEC-07114。
英文摘要：Insect cell lines have become useful tools in many research fields. Em
bryos of Musca domestica L. were used as experimental materials. The cells w
ere cultured in M3 medium containing 20% fetal bovine serum. After about 35day primar
y culture, the cells could be passaged one time every 7 days, and were stably pas
saged for 60generation period. The time of the cell population doublings was 4
4 h. The chromosomes are 2n=12. The cells from this cell line could be stored at
liquid nitrogen or 4℃ refrigerator, and resuscitated at room temperature. It was
named MDEC-07114.