短额负蝗精子发生过程中SBA受体的动态分布
Distribution of SBA receptors and microstructure of spermatogenic cells during spermatogenesis of Atractomorpha sinensis.
吕淑敏1**,赵 卓3,李建锋4,奚耕思2,3***
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作者单位：1西北农林科技大学 植物资源与病虫害治理教育部重点实验室 陕西 杨凌 712100 , 2陕西师范大学生命科学学院 西安 710062； 3吉林师范大学生命科学学院,四平 136000；4渭南师范学院科技处 陕西 渭南 714000
中文关键词：短额负蝗,精子发生，糖复合物，大豆凝集素
英文关键词：Atractomorpha sinensis, spermatogenesis, glycoconjugates, soybean agglutinin
中文摘要： 用常规组织学方法观察短额负蝗Atractomorpha sinensis Bolivar精子发生过 程中生精细胞的显微结构，并以大豆凝集素(soybean agglutinin, SBA)为探针利用凝集 素细胞化学方法研究该过程中N乙酰半乳糖复合物的分布变化。结果表明，短额负蝗精子 发生经历了精原细胞增殖期、初级精母细胞期、次级精母细胞期、精子细胞形成期和精子成 熟期5个时期，在这5个时期中各期生精细胞的大小、形态、核染色体等变化明显。在整个精 子发生过程中，N-乙酰半乳糖复合物出现于精原细胞期，并于精母细胞期发生明显的修饰 和变化，精子形成期和成熟期没有N-乙酰半乳糖复合物的表达。提示，N-乙酰半乳糖复合物的修饰和变化与短额负蝗生精细胞的生长和分化密切相关。
英文摘要： The microstructure of spermatogenic cells during spermatogenesis of Atractomor pha sinensis Bolivar was observed by the microscopic methods, and distribution of the soybean agglutinin (SBA) receptors during spermatogenesis was detected by us ing lectin-binding cytochemistry method. The results demonstrated that the proce ss of spermatogenesis in A. sinensis could be divided into 5 stages, includi ng t he spermospore proliferation stage, the primary spermatocyte stage, the secondar y spermatocyte stage, the spermateliosis stage and the spermiotiliosis stage. Du ring the whole process, great changes took place in size, morphology and chromos omes of the spermatogenic cells. The results of SBA labeling showed that the Gal NAc glycoconjugates first appeared at spermospore proliferation stage, and exper ienced obvious modification during the spermatocyte stage, but at the spermateli osis and the spermiotiliosis stage, the GalNAc glycoconjugates disappeared. Thes e results suggested that during the spermatogenesis of A. sinensis, the quan tity and location of the GalNAc glycoconjugates apparently changed, which may be res ponsible for the growth and differentiation of the spermatogenetic cells.