华北地区四种梢斑螟的遗传分化
Genetic divergence of four Dioryctria species from North China.
于潇翡1，杜艳丽2**，刘永杰1，张民照2，张 涛2
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作者单位：1山东农业大学植物保护学院 泰安 271018； 2北京农学院植物科学技术系 北京 102206
中文关键词：梢斑螟， 线粒体COⅠ基因， 遗传多样性， 系统发育
英文关键词：Dioryctria, COⅠ sequences, genetic diversity, phylogeny
中文摘要： 根据线粒体COⅠ基因序列，对华北地区梢斑螟属Dioryctria Zeller的微红梢斑螟D. rubella Hampson、芽梢斑螟D. yiai Mutuura & Munroe、大梢斑螟D. magnifica Munroe和冷杉梢斑螟D. abietella Denis & Schiffermüller共4种，9个不同地理 种群进行遗传多样性研究，并以同族的渣云翅斑螟Oncocera faecella (Zeller)为外 群探讨它们之间的系统发育关系。结果表明：(1)微红梢斑螟和大梢斑螟的亲缘关系较 近，与芽梢斑螟和冷杉梢斑螟构成的姐妹群分别形成华北地区梢斑螟属的两大支系；(2 )种内不同地理种群间的遗传距离(0.000～0.016)明显小于种间遗传距离(0.029～0. 089)，说明利用线粒体COⅠ基因序列研究梢斑螟属昆虫的系统发育关系是可行的 。
英文摘要： The phylogenesis of 9 geographical populations from 4 Dioryctria specie s, D. rubella Hampson, D. yiai Mutuura & Munroe, D. magnifica Munroe a nd D. abietella Denis & Schiffermüller were investigated based on COⅠ sequ e nces, with Oncocera faecella (Zeller) as outgroup. The genetic distances and phylogenetic tree revealed that:(1) the 4 Dioryctria species could be grouped into 2 clades. D．rubella and D. magnifica were clustered as one group, while D. yiai and D．abietella were clustered as the other; (2) the genetic distances b etween the geographic al populations were 0.000-0.016, significantly less than those between the s pecies (0.029-0.089), which indicated that COⅠ sequences may be suitable fo r determining the phylogenetic relationships of Dioryctria species.