江苏Q型烟粉虱抗性监测及其靶标基因克隆
Resistance monitoring and target gene cloning of Bemisia tabaci Q-biotype from Jiangsu Province
袁林泽1 王少丽2 陈雪林1 杜予州1 张友军2 王建军1**
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DOI：
作者单位：1.扬州大学园艺与植物保护学院 扬州 225009；2.中国农业科学院蔬菜花卉研究所 北京 100081
中文关键词：烟粉虱, 抗性监测, 乙酰胆碱酯酶, 钠离子通道, 靶标抗性
英文关键词： Bemisia tabaci,resistance monitoring,acetylcholinesterase,sodium channel,target-site associated resistance
中文摘要： 应用成虫浸叶生测法研究了江苏省扬州、无锡和东台3个地区Q型烟粉虱Bemisia tabaci(Gennadius)田间种群对5种杀虫剂的抗药性。结果表明，与相对敏感种群相比，Q型烟粉虱田间种群已对氯氰菊酯和吡虫啉产生了低到中等水平抗性，对阿维菌素仍然表现敏感。进一步通过RCR扩增获得了长度分别为287 bp和184 bp的烟粉虱乙酰胆碱酯酶ace1和para-同源钠离子通道基因片断。序列分析表明，江苏Q型烟粉虱存在与有机磷抗性相关的乙酰胆碱酯酶F331W突变和与拟除虫菊酯抗性相关的钠离子通道L925I和T929V突变。
英文摘要： The levels of resistance in three field populations of the
Bemisia tabaci Q-biotype from Yangzhou,Dongtai and Wuxi in Jiangsu Province to five representative insecticides were determined using the leaf dipping method.The result show that these three populations had low to moderate levels of resistance to cypermethrin and imidacloprid,but remain largely susceptible to abamectin compared to a relatively susceptible population.Two fragments of the
B.tabaci acetylcholinesterase enzyme ace1 and
para-type voltage gated sodium channel gene,with respective lengths of 287 bp and 184 bp,were amplified by PCR.Sequence analysis suggested the presence of the F331W mutation in acetylcholinesterase ace1 and L925I and T929V mutations in the sodium channel gene,mutations associated with resistance to organophosphate and pyrethroid insecticides,respectively.