小菜蛾卵表和雌蛾腹部鳞片提取液的GC-MS分析
Analysis of chemical components from eggs surface and female abdominal scales of Plutella xylostella
吕燕青 何余容** 陈科伟
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作者单位：华南农业大学昆虫学系 广州 510642
中文关键词：小菜蛾， GC-MS， 卵表， 鳞片， 分析
英文关键词：Plutella xylotella，GC-MS，egg surface，abdominal scales， analysis
中文摘要： 来源于小菜蛾Plutella xylostella (L.)卵表和雌蛾腹部鳞片的挥发性化学物质影响着卷蛾分索赤眼蜂Trichogrammatoidea bactrae Nagaraja、拟澳洲赤眼蜂Trichogramma confusum Viggiani、短管赤眼蜂Trichogramma pretiosum Riley和玉米螟赤眼蜂Trichogramma ostriniae Pang et Chen的搜索行为，为明确这些挥发性化学物质的主要成分，本文利用气相色谱-质谱联用仪（GC-MS）对小菜蛾卵表和雌蛾腹部鳞片正己烷提取液的化学成分进行分析。结果总共分离到48种挥发性化学物质，其中卵表中16种，雌蛾腹部鳞片中32种。经NIST数据库检索，并与标准图谱比较，应用色谱峰面积归一法测定各成分的相对含量,结果表明，鉴定出卵表中有11种化学物质，占其总挥发性组分总量的93.95%；雌蛾腹部鳞片中有17种化学物质，占其总挥发性组分总量的88.47%。结果表明，小菜蛾卵表和雌蛾腹部鳞片中的主要成分为12~36碳的直链和支链饱和烷烃及一些脂肪酸衍生物。
英文摘要： Volatile chemicals emanating from the eggs and adult abdominal scales of the Diamondback moth (DBM),Plutella xylostella (L.),influence the host-seeking behavior of Trichogrammatoidea bactrae Nagaraja,Trichogramma confusum Viggiani, Trichogramma pretiosum Riley and Trichogramma ostriniae Pang et Chen.In order to identify the main component of the volatile chemicals emitted by DBM eggs and adults,the hydrocarbon profile of hexane extracts collected from the surface of DBM eggs and female abdominal scales were analyzed using Gas Chromatography/Mass Spectrometry (GC-MS).Forty-eight chemical components were isolated,16 components from the eggs’ surface and 32 from female abdominal scales.The contents of these chemicals was determined by the area normalizing method after searching the NIST database and comparison with standard mass spectra.Eleven chemical components from the eggs’ surface were identified with a total mass fraction of 93.95%.Seventeen chemical components from female abdominal scales were identified with total mass fraction of 88.47%.The principal components of hexane extracts from the surface of DBM eggs and female abdominal scales were straight and branched-chain saturated hydrocarbons ranging from C-12 to C-36 with some aliphatic acid ramification.