西花蓟马表皮碳氢化合物成份分析
Analysis of cuticular hydrocarbons of Frankliniella occidentalis
赵成银1,2,何余容2,钟锋1,2,高燕1,齐国君1,邵晓迎1,吕利华1 ** 齐国君 等
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作者单位:1广东省农科院植物保护研究所广州510640; 2华南农业大学昆虫学系广州510642
中文关键词:西花蓟马,表皮碳氢化合物,GC-MS,分析
英文关键词:Frankliniella occidentalis, cuticular hydrocarbons, GC-MS, analysis
中文摘要: 西花蓟马Frankliniella occidentalis(Pergande)是一种重要的世界性害虫。为了明确西花蓟马表皮碳氢化合物的主要成分,本文采用改进的进样技术,利用气相色谱-质谱联用仪(GC-MS)对西花蓟马成虫和若虫表皮碳氢化合物进行分析。经NIST数据库检索,并与标准图谱比较,应用色谱峰面积归一法测定各种成分及其相对含量,实验结果表明,西花蓟马成虫和若虫表皮中的主要成分为C25~C29的直链和支链饱和烷烃;成虫表皮中含有9种碳氢化合物,若虫表皮中有8种碳氢化合物;9-甲基二十五烷是其成虫特有的,其它8种碳氢化合物是成虫与若虫共有的,但其含量略有差异。
英文摘要: The western flower thrip (WFT) Frankliniella occidentalis (Pergande) is a serious pest responsible for causing substantial economic losses in a broad range of crops worldwide.The cuticular hydrocarbons from the cuticle of WFT were analyzed by gas charomatography and mass spectrometry (GC-MS) without solvent.The composition of the cuticular hydrocarbons was then determined by the area normalizing method.Searching the NIST database and comparison with standard mass spectra identified nine constituents in the cuticular hydrocarbons of adults, and eight in those of nymphs.With the exception of 9-methylpentacosane, which was detected only in adults, the same hydrocarbon patterns were found in both adults and nymphs.The principal components of WFT cuticular hydrocarbons were straight and branchedchain-saturated hydrocarbons ranging from C25 to C29.