（安徽农业大学植物保护学院合肥230036）摘要从褐飞虱Nilaparvata lugens（Stl）罹病虫体上新分离出的一种黄绿绿僵菌菌株Metarhizium flavoviride（Mf82）,与实验室保存的黄绿绿僵菌、金龟子绿僵菌和白僵菌3种菌种5个菌株作为对比，研究了在SDAY和PDA培养基上的培养性状，并测定了它们对褐飞虱成虫的毒力。结果表明：在SDAY培养基上，6个菌株菌落特征差异明显，Mf82菌株菌落直径增长量最大，产孢量最高，分别为259 mm，1734× 107孢子/ cm2，与其它菌株相比差异显著。Mf82菌株在SDAY培养基上生长优于PDA， Mf82菌株对褐飞虱成虫具有很强的毒力，以10×108孢子/ mL 的孢子液接种到褐飞虱成虫体表上，累积校正死亡率达838%，LT50为447 d，致死率明显高于其他真菌。Mf82菌株在褐飞虱的生物防治中很有应用前景。
关键词黄绿绿僵菌， 培养性状， 褐飞虱， 毒力

Culturing characteristics of entomogenous fungi strains and
their virulence to Nilaparvata lugens（Stl） ZHANG SongYing** LIN HuaFeng***LI MaoYeJIN Li
(School of Plant Protection, Anhui Agricultural University, Hefei230036, China)

AbstractSix strains of entomogenous fungi; a new strain, Metarhizium flavoviride Mf82, isolated from Nilaparvata lugens （Stl） and two strains of Beauveria bassiana and three of Metarhizium, were cultured on SDAY and PDA and their virulence to adults of N.lugens compared. There were obvious differences in the growth of the six strains on SDAY; Mf82 grew faster and produced more spores than the other strains with a colony diameter growth rate of 2.59 mm per day and sporulation of 17.34× 107 conidia/ cm2. SDAY was therefore a more suitable medium for Mf82 than PDA. Mf82 clearly controlled N. lugens adults; the LT50 value was 4.47 d and adjusted accumulative mortality was 83.8% at a concentration of 1.0×108conidia/ml. Mf82 is therefore a promising biological pesticide for the control of N. lugens.
Key wordsMetarhizium flavoviride, cultural characteristics, Nilaparvata lugens, virulence