Vip3Aa及Cry1Ac对棉铃虫幼虫多种酶活力的影响
Effects of Vip3Aa and Cry1Ac on enzyme activity in cottonbollworm Helicoverpa armigera larvae
张 彦 梁革梅 高 珍
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作者单位：中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室北京100193
中文关键词：棉铃虫，Vip3Aa，Cry1Ac，蛋白酶，解毒酶
英文关键词：Helicoverpa armigera，Vip3Aa，Cry1Ac，protease，detoxification enzymes
中文摘要： 为了明确Vip3Aa 的作用机制，为其作为新毒素策略重要蛋白的应用提供理论依据，本文比较了Vip3Aa、
Cry1Ac 对棉铃虫Helicoverpa armigera(Hübner)主要蛋白酶、解毒酶、APN 活性的影响，并研究了Vip3Aa 和Cry1Ac共同使用对几种酶活力的作用。室内生测结果表明，Vip3Aa 对棉铃虫的杀虫效果低于Cry1Ac，但Vip3Aa 对棉铃虫幼虫生长有明显的抑制作用。取食含Cry1Ac、Vip3Aa 或Cry1Ac + Vip3Aa 饲料的棉铃虫，总蛋白酶和类胰凝乳蛋白酶活性很快升高;但经Cry1Ac 处理12 h 后这2 种酶活性与对照差异不显著或低于对照，而取食含Vip3Aa 饲料的棉铃虫酶活力显著高于对照的时间明显延长，而且类胰蛋白酶活性也显著高于对照;表明Cry1Ac 降解速度
比Vip3Aa 快，可能是由于降解2 种蛋白参与的酶系存在差异，同时Cry1Ac + Vip3Aa 混用可以延长蛋白被酶解的
时间。谷胱甘肽S-转移酶和α-乙酸萘酯酶活性在棉铃虫取食含Vip3Aa、Cry1Ac 或Cry1Ac + Vip3Aa 蛋白的饲料
后活性升高，说明这2 种酶可能参与了对Cry1Ac、Vip3Aa 的解毒作用。但Cry1Ac、Vip3Aa 对氨肽酶活性影响不
大，可能在毒蛋白发挥毒性的过程中与氨肽酶活力变化无关。
英文摘要：In order to clarify the activity of Vip3Aa and provide a theoretical basis for its application in the“New toxin
strategy”，the effects of Vip3Aa and Cry1Ac on protease，detoxification enzymes were and aminopeptidase N ( APN)
activity in larvae of the cotton bollworm Helicoverpa armigera ( Hübner) were compared and the impacts of Cry1Ac +
Vip3Aa on these enzymes were investigated． The lethality of Vip3Aa was lower than that of Cry1Ac，but Vip3Aa had an
obvious inhibitory effect on larval development． Total protease and trypsin-like enzyme activity quickly increased in H．
armigera fed an artificial diet containing either Cry1Ac or Vip3Aa or Cry1Ac + Vip3Aa． However，compared to the
control，there was no significant difference in the activity of these two enzymes after 12 h of feeding on the Cry1Ac diet．
While the period during which the activity of these two enzymes increased was clearly prolonged in larvae fed on diets
containing Vip3Aa，that of the chymotrypsin-like enzyme was also higher than in the control． This indicates that the
degradation rate of Cry1Ac was faster than that of Vip3Aa，and that the enzyme systems involved in degradation could be
different． Meanwhile，the diet containing Cry1Ac and Vip3Aa together extended the degradation time． The activities of
glutathione-S-transferase and α-naphthalene acetate esterase increased in H． armigera fed on a diet containing either
Cry1Ac or Vip3Aa or Cry1Ac + Vip3Aa． This indicates that these enzymes may be involved in the detoxification of Vip3Aa
and Cry1Ac． However，Vip3Aa and Cry1Ac had little effect on aminopeptidase N activities，suggesting that the toxicity of
the latter has no relationship to APN enzyme activity．