转基因棉Bt毒蛋白含量与抗棉大卷叶螟效果间的关系分析
The relationship between Bt toxin content and resistance toSylepta derogata in various transgenic Bt cotton varieties
肖留斌 柏立新 谭永安
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作者单位：江苏省农业科学院植物保护研究所南京210014
中文关键词：棉大卷叶螟，转基因棉，抗性，Bt 毒蛋白
英文关键词：Sylepta derogate，transgenic cotton，resistance，Bt toxin protein
中文摘要：棉大卷叶螟Sylepta derogata Fabricius 为近年来长江流域棉花中后期的一种重要害虫。在其危害盛期测
定了8 种转基因棉叶片Bt 毒蛋白的含量与受害程度，在此基础上就转基因棉对棉大卷叶螟的抗虫性采用不同抗
性指标相结合的方法进行了综合评估，同时对转基因棉对棉大卷叶螟的抗虫效果与毒蛋白含量的相关性进行分
析。结果表明:各转基因棉品种的不同部位叶片的毒蛋白含量呈现顶叶最高，功能叶次之，老叶含量最低的趋势。
抗蚜8017 和SGK321 2 个转基因棉品种的棉叶毒蛋白表达量相对较低，平均值在120 μg / g 以下，对棉大卷叶螟的
抗性级别均为中抗。其它供试转基因棉棉叶毒蛋白表达量均值在150 μg / g 以上，抗性级别也均为高抗，而非转基
因棉泗棉3 号与石远321 对棉大卷叶螟不具抗性。转基因棉对棉大卷叶螟的抗性水平与毒蛋白含量呈正相关。
英文摘要：In recent years，Sylepta derogata Fabricius has been an important insect pest of late growth stage cotton in the
Yangtze River Region． In order to study the relationship between Bt toxin levels and resistance to S． derogata in transgenic
cotton，the Bt toxin content of the leaves of eight Bt cotton varieties was assayed and the resistance of each variety to S．
derogata comprehensively evaluated． The results show that the Bt toxin content of the leaves of Kangya-8017 and SGK321
were less than 120 μg / g and the resistance of these two varieties to S． derogate was moderate． The other 6 varieties of Bt
cotton were highly resistant to S． derogate and had toxic protein contents of ＞ 150 μg / g． The parental non-transgenic
cotton variety did not show any resistance to S． derogate． These results indicate that the resistance of Bt transgenic cotton
to S． derogata is related to the level of Bt toxin in the plant．