香蕉假茎象甲对不同生理状态香蕉假茎挥发物的行为反应
Study of the behavioral responses of Odoiporus longicollis to volatiles from banana pseudostems with different physiological status
尹 炯 赵冬香 王玉洁 高景林 卢芙萍 等
点击：2079次 下载：6次
DOI：
作者单位：中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室儋州571737
中文关键词：香蕉假茎象甲，行为反应，香蕉假茎，挥发物
英文关键词： Odoiporus longicollis，behavioral response，banana pseudostem，volatiles
中文摘要：采用固相微萃取技术提取新鲜假茎、腐烂未虫蛀假茎、虫蛀且腐烂假茎以及虫蛀未腐烂假茎4 种生理状
态的香蕉假茎挥发物，进行气质联用仪分析鉴定。结果表明:从香蕉新鲜假茎与虫蛀且腐烂假茎中分别鉴定出10
种挥发性物质;腐烂未虫蛀假茎与虫蛀未腐烂假茎分别鉴定出11 种挥发性物质;这些挥发物包括烃类、酯类、酮
类、杂环类等，在不同生理状态的香蕉假茎中的相对含量各不相同。4 种生理状态的香蕉假茎挥发性成分种类数
及相对含量的变化与香蕉假茎象甲Odoiporus longicollis Olivier 的为害有着密切关系，遭受香蕉徦茎象甲为害的香
蕉假茎，其共同成分2，6 － 二甲基－ 2，4，6 － 辛三烯相对含量对应地减少。利用双重陷阱嗅觉仪测定了香蕉假茎
象甲对香蕉假茎挥发物的行为反应，结果表明:4 种生理状态的香蕉假茎挥发物均对香蕉假茎象甲有引诱作用，与
新鲜假茎相比，香蕉假茎的虫蛀且腐烂状态可增强其对香蕉假茎象甲雌、雄虫的引诱作用。
英文摘要：Solid phase microextraction ( SPME) was used to extract volatiles from banana pseudostems in the following
four physiological states:fresh，rotten but not fed on by beetles，rotten and fed on by beetles，and fresh and fed on by
beetles． The extracted volatiles were analyzed by gas chromatography-mass spectrometry ( GC-MS ) ． A total of 10
components were identified from volatiles extracted from fresh and rotten pseudostems that had been fed on by beetles，
whereas a total of 11 components were identified from extracts from rotten pseudostems and fresh pseudostems that had
been fed on by beetles． The extracted volatiles included hydrocarbon，esters，ketone and heterocyclic compounds，the
relative abundance of which differed with pseudostems’physiological state． The species and relative abundance of
extracted volatiles were closely related to damage inflicted by the banana pseudostem weevil． There was a corresponding
reduction in the relative content of the common component allo-ocimene after Odoiporus longicollis Olivier infestation． The
behavioral responses of male and female O． longicollis to extracted volatiles from banana pseudostems in each of the four
physiological states were observed with a double pitfall olfactometer． Both male and female O． longicollis were attracted to
the volatiles from banana pseudostems in different physiological states． Compared to fresh pseudostems，beetle feeding
activity and rottenness enhanced the attractiveness of the extracted volatiles from banana pseudostems to male and female
O． longicollis．