转Bt-cry1Ah基因玉米花粉对意大利蜜蜂幼虫的影响
Effects of Bt-cry1Ah corn pollen on larvae of Apis mellifera ligustica
代平礼1**,周玮1,张杰2,郎志宏3,周婷1***,王强1,崔红娟2,姜玮瑜2,吴艳艳1
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作者单位：1. 中国农业科学院蜜蜂研究所/农业部授粉昆虫生物学重点开放实验室北京100093；2. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室北京100193；3.中国农业科学院生物技术研究所北京100081
中文关键词：蜜蜂，幼虫，转基因玉米，cry1Ah 基因，花粉
英文关键词：honeybee，larvae，transgenic corn，cry1Ah gene，pollen
中文摘要：新型杀虫蛋白基因cry1Ah 基因是中国农业科学院植物保护研究所从Bt 菌株BT8 中鉴定克隆的，其编码
蛋白对鳞翅目害虫具有强毒力，尤其对亚洲玉米螟Ostrinia furnacalis(Guenée)的毒力强于目前使用的cry1A 类基
因。转cry1Ah 基因抗虫玉米具有很好的应用前景。花粉是蜜蜂重要的食物来源，蜜蜂是转基因植物安全性评价
的关键测试生物。因此，开展转cry1Ah 基因玉米对蜜蜂的安全性研究很有必要。给意大利蜜蜂Apis mellifera
ligustica Spinola 蜂群中4 ～ 6 日龄幼虫饲喂转基因玉米花粉、常规玉米花粉、杂花粉，哺育蜂饲喂为对照。转基因
玉米花粉对意大利蜜蜂封盖率、出房率和发育历期没有显著影响。表明转cry1Ah 基因玉米花粉对意大利蜜蜂幼
虫的存活和发育没有不良影响。
英文摘要：The cry1Ah gene，which displays high toxicity against Lepidopteran larvae，was one of the novel insecticidal
genes cloned from Bacillus thuringiensis isolate BT8． Cry1Ah protein have higher toxicity to the Asian corn borer (Ostrinia
furnacalis(Guenée) ) than any other cry1A genes． The cry1Ah gene was therefore a candidate gene for insect resistant
transgenic corn research． Pollen is a significant component of the diet of honeybees． Thus the honeybee may serve as one
of the key species to be tested for the potential adverse effects of transgenic crops and assessment of impacts on honeybees
is an essential part of the risk assessment process for Bt cry1Ah gene corn． The effects of dietary transgenic Bt corn pollen
on honeybee worker larvae of Apis mellifera ligustica Spinola was examined． We measured cap rate，emergence rate，and
immature stage after 4-6-day-old larvae were fed either cry1Ah corn pollen，regular corn pollen，mixed bee pollen and a
control． There were no significant differences in all the parameters measured between bees fed these diets． These results
suggest that transgenic Bt corn pollen does not pose a threat to honeybee larval development．