蜜蜂体内王浆主蛋白基因mrjp9的转录表达研究
Transcription analysis of mrjp9 in the honeybee Apis mellifera ligustica
李江红**,刘振,陈大福,梁勤***
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作者单位：福建农林大学蜂学学院福州350002
中文关键词：蜜蜂，mrjp9，转录，行为，发育
英文关键词： honeybee，mrjp9，transcription，behavior，development
中文摘要：蜜蜂体内有9 种王浆蛋白基因(major royal jelly protein，MRJPs1 ～ 9)，其中MRJPs1 ～ 5 在蜂王浆中含量
较高，是蜂王浆生物学功能的基础。MRJPs6 ～ 9 在王浆中没有或含量极少，且功能未知。为研究非王浆蛋白组分
的MRJP9 的生物学功能，本研究用RT-PCR 的方法对意大利蜜蜂Apis mellifera ligustica Spinola 不同组织，不同部
位，不同级型样本中mrjp9 的转录水平进行检测和定量。结果发现mrjp9 在蜜蜂的幼虫、蛹和成年蜜蜂的各组织部
位均广泛转录表达，但其在幼虫、蛹和刚出房的成年蜜蜂体内表达水平较低，而在成年采集蜂体内表达水平则较
高，其表达与蜜蜂的发育时期有关。通过对在成年蜜蜂体内各组织部位的表达水平进行检测的结果显示该基因
主要在蜜蜂的头、胸和王浆腺等组织部位的表达较高，其他组织部位表达较少。此外，该基因也在雄蜂和蜂王体
内广泛表达，不受蜜蜂性别和级型的影响。这些结果说明mrjp9 是一与蜜蜂发育有关的基因，可能与蜜蜂的行为
发育和分工调控有关。
英文摘要：Nine major royal jelly proteins (MRJPs1-9) were identified in the honeybee genome． Among these，MRJPs1-
5 were the main components of royal jelly protein and the functional basis of royal jelly． MRJPs6-9 are either not present，
or are less abundant，in royal jelly and their function remains unclear． To explore the possible function of MRJP9，Total
RNA was isolated from different body parts of honeybees at different developmental stages，and the expression of mrjp9
determined by RT-PCR． The results show that mrjp9 was widely expressed in all parts of honeybee at all developmental
stages． However，the level of mrjp9 expression was relatively low in larvae，pupae and early emerged adults，but relatively
high in foraging honeybees． Expression of mrjp9 was therefore related to developmental stage． In foraging honeybees，
expression of mrjp9 was particularly high in the head，thorax and hypopharyngeal gland，but less in other body parts or
tissues． Furthermore，mrjp9 was also expressed in multiple tissues of drone and queen honeybees and was not related to
sex or caste． The profile of mrjp9 expression coincided with honeybee behavioral development; expression was high in the
head and thorax which are the body parts directly related to foraging activity． Therefore，mrjp9 may be involved in the
behavioral development and division of labor in the honeybee．