百草枯对蜜蜂寿命及抗氧化酶基因表达影响
Effects of paraquat on lifespan and level of expression of antioxidantenzyme genes in worker bees of Apis mellifera ligustica
王文祥**,王欢,张飞,吴小波,曾志将***
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作者单位：江西农业大学蜜蜂研究所南昌330045
中文关键词：意大利蜜蜂，工蜂，寿命，抗氧化酶基因
英文关键词：Apis mellifera ligustica，worker，lifespan，antioxidant enzyme
中文摘要： 本研究用200、400 和600 mg /L 的百草枯饲喂刚出房的意大利蜜蜂Apis mellifera ligustica Spinola 工蜂，检
测其对工蜂寿命及抗氧化酶基因Sod 1 和Sod 2 表达的影响，并以饲喂蔗糖作为对照组。结果表明:随着百草枯浓
度的增加，工蜂的寿命极显著下降( P ＜ 0. 01) ;抗氧化酶基因Sod 1 的相对表达量，随着百草枯浓度的增加而增
加，其中对照组Sod 1 基因的相对表达量显著低于各百草枯处理组( P ＜ 0. 05) ;Sod 2 基因的相对表达量，随着百
草枯浓度的增加，先增加后减少，其中600 mg /L 百草枯处理组Sod 2 基因的相对表达量显著低于对照组和低剂量
百草枯处理组(P ＜ 0. 05)。
英文摘要： We investigated the effects of paraquat on lifespan and the level of expression of mRNA for the antioxidant enzyme genes Sod 1 and Sod 2 in newly emerged worker bees of Apis mellifera ligustica Spinola． Paraquat was given atdoses of 200 mg /L，400 mg /L and 600 mg /L． Bees in the control group were fed sucrose． The results showed thatlifespans of honey bees decreased with the increase of paraquat concentration(P ＜ 0. 01) ． The relative level of expressionof the antioxidant enzyme gene Sod 1 increased linearly with the increase of paraquat concentration and it was significantlylower in the control group than in the paraquat-treated groups (P ＜ 0. 05) ． In contrast，the relative level of expression ofthe antioxidant enzyme gene Sod 2 increased initially but then decreased with the increase of paraquat concentration． The level of expression of this gene was significantly lower in the high-dosage paraquat-treated group (600 mg /L) than that inboth the control group and the low-and middle-dosage paraquat-treated groups (P ＜ 0. 05) ．