苹果蠹蛾线粒体DNA-COI基因克隆与序列分析
Cloning and sequencing analysis of the codling mothCydia pomonella COⅠ gene
冯宏祖 王兰 郭文超 曹玉 杨明禄 等
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作者单位：1. 塔里木大学植物科学学院阿拉尔市843300;2. 新疆农业科学院植物保护研究所乌鲁木齐市830091;
中文关键词：果蠹蛾，COⅠ基因，克隆，序列分析
英文关键词：codling moth，Cydia pomonella，COⅠ gene，clone，sequence analysis
中文摘要： 本研究采集新疆阿拉尔地区苹果蠹蛾Cydia pomonella( L． )幼虫，对其线粒体DNA 细胞色素氧化酶Ⅰ亚基(COⅠ)基因进行了扩增、克隆和测序，并对COⅠ序列进行了分析。结果显示:苹果蠹蛾DNA 扩增出的COⅠ基因序列片段长度为709 bp，序列中A + T 含量极高，占68. 7% ，而G + C 的含量只有31. 3% 。经基因序列比对，与其它几种食心虫的同源性为85. 4% ～ 88. 1% ，遗传距离为0. 130 ～ 0. 162;采用NJ 法构建了卷蛾科系统树，所得的聚类结果与传统的分类结果基本一致。本研究结果为苹果蠹蛾快速鉴定的DNA 条形码技术研究提供重要基础。
英文摘要：
The mitochondrial COⅠ gene fragment of codling moth Cydia pomonella ( L． ) specimens from the Alar area ofXinjiang was amplified via PCR and the PCR products were purifiedclonedandsequenced． 709 bp nucleotide sequencesere obtained with a very high A + T content (68. 7% ) and relatively low G + C ontent (31. 3% ) ． Comparing the COⅠsequence of the codling moth with that of several other moth species，the homologies of nucleotides ranged from 85. 4% to88. 1% and the genetic distances ranged from 0. 130 to 0. 162. Reconstructing the molecular phylogenetic tree of theTortricidae with the Neighbor-Joining ( NJ ) method produced a result in accord with the raditional morphologicalclassification． The results of this study provide an importantbasisfortherapididentification of C． pomonella with DNAbarcode technology．