亚洲玉米螟不同地理种群基于COI基因序列初步分析
Variation of mtDNA COⅠ sequences in different geographicpopulations of the Asian corn borer，Ostrinia furnacalis
黄丽莉 阙海勇 陈超 匡先钜 薛芳森
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作者单位：1. 江西农业大学昆虫研究所南昌330045; 2. 江西出入境检验检疫局南昌330002)
中文关键词：亚洲玉米螟，mtDNA-COⅠ，地理种群，遗传分化
英文关键词： Ostrinia furnacalis，mtDNA-COⅠ，geo-population，genetic differentiation
中文摘要： 本研究通过对我国亚洲玉米螟Ostrinia furnacalis (Guenée)6 个地理种群的线粒体DNA COⅠ基因进行测序，运用分子进化遗传分析软件MEGA5 对序列进行对比和同源性分析，并建立系统发育关系。结果表明，COⅠ基因在6 个地理种群中进化具有一定的差异，平均遗传距离为0. 013;进化树显示，亚洲玉米螟的三亚种群、广州种群与南昌种群聚为一支，然后与泰安种群聚为一支，再与哈尔滨种群聚为一支;而廊坊种群另聚成一支。遗传分歧度与系统发育进化关系均表明不同地理种群的遗传分化与地理距离之间具有一定的相关性。
英文摘要： The mitochondrial DNA ( mtDNA) cytochrome oxidase subunitⅠ( COⅠ) of 6 geo-populations of Ostrinia
furnacalis ( Guenée ) was sequenced． The identity and variation of the resultant sequencesandtheirphylogenicrelationships were established by Mega 5. The results show that there are some differences in COⅠ sequences amongdifferent geo-populations of O． furnacalis and that the average evolutionary divergence between populations is 0. 012.Molecular phylogeny showed that theSanyanGuangzhou and Nanchang populations formed one branch，and the Taian，Haerbin and Langfangpopulations formed separate branches． The results of evolutionary divergenceandmolecularphylogeny suggest that the separation of different geo-populations of O． furnacalis is，at least to some extent，relative togeographic distance．