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嗡蜣螂属Onthophagus十二种蜣螂线粒体COⅠ基因的DNA条形码研究
Application of DNA barcoding to mtDNA COⅠ genes in 12 dung beetle (Onthophagus) species
李蓓1,郭晓华1** ,刘广纯1,刘新民2
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DOI:
作者单位:1.辽宁省城市有害生物治理与生态安全重点实验室沈阳大学生物与环境工程学院沈阳110044;2.内蒙古师范大学 生命科学与技术学院呼和浩特010022
中文关键词:DNA条形码, 嗡蜣螂属, COⅠ基因, 分类鉴定
英文关键词:DNA barcodes, Onthophagus, COⅠ gene, taxonomy
中文摘要:       对嗡蜣螂属Onthophagus 12种蜣螂的线粒体COⅠ基因3′端部分序列(731 bp)进行了比较,结果显示,COⅠ序列的变异位点213个,简约信息位点167个。碱基替代主要发生在第3位点(64次),占替代总数的83.12%。除掘嗡蜣螂O. fodiens与婪嗡蜣螂O. lenzi小于2%外,其余种间遗传距离在8.1%~15.8%之间,种内遗传距离为0~0.2%。单倍型多样性(Hd)和核苷酸序列多样性(Pi)分别为 0.944±0.030和0.10518± 0.0045。滑动窗口分析表明,可变位点频率在240~290 bp、675 bp附近较高。NJ树聚类结果与传统形态学分类相吻合:外群代表种分化最早,种间聚成一分支,种内个体优先聚集种下。本文认为COⅠ基因适合作为嗡蜣螂属物种鉴定的DNA条形码。
英文摘要:         The DNA sequences of 12 dung beetle species from the genus Onthophagus were compared using a molecular marker of the 3′end fragment(731 bp) of the mitochondrial DNA COⅠ gene. 213 variable sites and 167 parsimony information sites were identified. Base substitution mainly occurred on the third codon (64 times), accounting for 83.12% of all substitutions. Interspecific divergence spanned from 8.1% to 15.8%, with the exception of the genetic distance between O. fodiens and O. lenzi which was below 2%, and intraspecific variation varied from 0 to 02%. Haplotype diversity (Hd) and nucleotide diversity (Pi) were 0.944±0.030 and 0.10518± 0.0045, respectively. Two sliding windows analyses demonstrated that the frequency of variable sites for all sequences located within 240-290 bp and approximately 675 bp was high. NJ phylogenetic tree revealed that molecular clusters was consistent with the traditional taxonomy based on morphological characteristics. The outgroup lineages separate first from the phylogenetic tree. Interspecific specimens clustered in one branch with intraspecific specimens clustered according to species and that branch. These results indicate that COⅠgene barcoding can reliably identify species of the genus Onthophagus.
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