大蜡螟中类肽聚糖识别蛋白Gm21的鉴定、基因克隆及序列分析
Identification, cDNA cloning and sequence anlysis of a peptidoglycanrecognitionlike protein Gm21 in Galleria mellonella
石怀兴**,曾洪梅,邱德文***
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作者单位：中国农业科学院植物保护研究所 农业部有害生物综合治理重点实验室北京100081
中文关键词：大蜡螟，肽聚糖识别蛋白，免疫
英文关键词：Galleria mellonella, peptidoglycanrecognition protein, immune
中文摘要： 为了调查毒素蛋白免疫下大蜡螟Galleria mellonella L.产生的免疫相关蛋白，采用双向电泳的方法，从大蜡螟血淋巴中鉴定得到一种昆虫肽聚糖识别蛋白Gm21，通过RTPCR、cDNA末端快速扩增 (RACE) 技术克隆得到其全长基因，该基因全长cDNA具有完整的编码框，编码211个氨基酸，序列分析表明该蛋白是一种具有潜在酰胺酶活性的S型肽聚糖识别蛋白。本研究中Gm21蛋白在免疫压力下的上调表达及其cDNA序列的克隆，对进一步深入研究昆虫肽聚糖识别蛋白的功能具有重要的意义。
英文摘要： An investigation was made of the immune protein in Galleria mellonella L. that had been immunechallenged with toxic protein. A peptidoglycanrecognition protein, Gm21, in hemolymph of G.mellonella was identified by two dimensional (2\|D) electrophoresis, and the encoding gene was cloned by RTPCR and RACE. The full length cDNA has the complete open reading frame and encodes a protein of 211 amino acids. Sequence analysis indicated that the protein is a peptidoglycanrecognition protein (PGRP) \|Slike protein with potential amindase activity. The increased expression of Gm21 protein in immunechallenged G. mellonella and molecular cloning of the Gm21 gene are important techniques for further study of peptidoglycanrecognition proteins.