抗Cry1Ac小菜蛾种群生命表及其适合度研究
Life table and relative fitness of a strain Plutella xylostella resistant to Bacillus thuringiensis Cry1Ac toxin
秦悦,朱勋,雷妍圆,郑晓旭,吴青君,王少丽,张友军**
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作者单位：中国农业科学院蔬菜花卉研究所北京100081
中文关键词：小菜蛾，Cry1Ac毒蛋白，生命表，相对适合度
英文关键词：Plutella xylostella, Cry1Ac toxin, life table, relative fitness
中文摘要： 本实验研究了小菜蛾Plutella xylostella L.高抗Cry1Ac种群DBM1Ac-R（抗性倍数大于1000）及敏感种群DBM1Ac-S的生长发育、繁殖等生物特征情况，组建了两种群的生命表，并对其适合度进行了研究。结果表明，DBM1Ac-R的产卵量、卵历期、孵化率和化蛹率、蛹重及雌雄比(♀:♂)，均显著低于DBM1Ac-S。 DBM1Ac-R相对于DBM1Ac-S的相对适合度为0.5762，表明Cry1Ac抗性种群在繁殖能力上存在明显的生存劣势。
英文摘要： A resistant strain (DBM1AcR) of Plutella xylostella L. (diamondback moth) with more than 1 000fold resistance to Cry1Ac was obtained. Life history characteristics, such as the development and fecundity of susceptible (DBM1AcS) and resistant strains, were studied. Life tables were established and the relative fitness cost of developing resistance analyzed. The results show that the DBM1AcR strain has distinct reproductive disadvantages, such as a fitness value of 0.5762, including lower fecundity, hatching rate, pupation rate and sex ratio (♀:♂), shorter egg period and lighter pupa weight, compared with the nonresistant DBM1AcS strain.