西花蓟马对吡虫啉的抗性机制及交互抗性研究
Mechanisms and crossresistance of imidacloprid resistance in Frankliniella occidentalis
王圣印1,2,周仙红1,张安盛1,李丽莉1,门兴元1,刘永杰2,李许可2,于毅1**
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作者单位：1.山东省农业科学院植物保护研究所山东省植物病毒学重点试验室济南250100；2．山东农业大学植物保护学院泰安271018
中文关键词：西花蓟马， 交互抗性， 羧酸酯酶， 谷胱甘肽S\|转移酶
英文关键词：Frankliniella occidentalis, crossresistance, carboxylesterase, glutathione Stransferase
中文摘要： 为了解西花蓟马Frankliniella occidentalis (Pergande)对吡虫啉的抗性风险，本文就吡虫啉的交互抗性和抗性机制（增效剂和酶活性）进行了研究。结果表明，经过35代筛选，西花蓟马对吡虫啉的抗性上升到21.26倍。西花蓟马对吡虫啉与阿维菌素和甲维盐存在中等水平交互抗性，与氯氟氰菊酯、灭多威和毒死蜱存在低水平交互抗性。增效剂试验表明，三丁基三硫磷酸酯（DEF）、磷酸三苯酯（TPP）和马来酸二乙酯（DEM）具有显著增效（SR50,DEF=6.38，SR50,TPP=5.52，SR50,DEM=1.60，P<0.05）。生化测定表明：抗吡虫啉品系西花蓟马的羧酸酯酶（5.06倍）和谷胱甘肽S\|转移酶酶活性（1.63倍）均显著（P<0.05）高于敏感品系，表明羧酸酯酶和谷胱甘肽S-转移酶酶活性的提高是西花蓟马对吡虫啉产生抗药性的主要原因。
英文摘要： In order to better understand the risk of imidacloprid resistance in Frankliniella occidentalis (Pergande), crossresistance and resistance (synergists and enzyme activity) of this pesticide were investigated. After 35 generations’ selections, the selection populations obtained 2126\| fold resistance to imidacloprid. Imidacloprid had medium levels of crossresistance to abamectin and emamectin benzoate, and had low levels of crossresistance to cyhalothrin, methomyl and chlorpyrifos. The synergists s,s,stributyl phosphorotrithioate(DEF), triphenyl phosphate(TPP) and diethyl maleate (DEM) showed significant synergism (SR50,DEF=638, P<0005; SR50,TPP=552，P<0005; SR50,DEM=160，P<0005) with regard to the toxicity of imidacloprid in the resistant population (BK). In vitro assays of enzyme activities showed significantly increased activity of carboxylesterase (506
\|fold) and glutathione Stransferases (163\|fold) in the resistant population (BK), indicating that enhanced detoxification is responsible for imidacloprid resistance in F. occidentalis.