二斑叶螨对阿维菌素敏感性的快速检测方法筛
A rapid test to determine the sensitivity of Tetranychus urticae to abamectin
马惠1**,刘庆娟3,张安盛2,韩凤英4,张毓妹5,周仙红2,于毅2***
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作者单位:1山东省农业科学院棉花研究中心济南250100;2山东省农业科学院植物保护研究所 山东省植物病毒学重点实验室济南250100;3山东农业大学植物保护学院泰安271018;4山东省农业管理干部学院现代农业技术系济南250100;5河北农业大学植物保护学院保定071000
中文关键词:二斑叶螨,阿维菌素,敏感性,快速检测方法
英文关键词:Tetranychus urticae, abamectin, sensitivity, rapid determination method
中文摘要:为了寻找一种快速检测二斑叶螨Tetranychus urticae Koch对阿维菌素敏感性的试验方法,本试验分别采用酶标板法、玻璃管法、离心管法、
玻片浸渍法和培养皿封膜法,测定了阿维菌素处理后1、2、3 和4 h时二斑叶螨的死亡率。结果发现,酶标板法和玻璃管法操作不方便;离心
管法无法准确判断死亡与否;玻片浸渍法作用速度慢;培养皿封膜法方便快捷。据此得出,采用培养皿封膜法于处理后3 h观察结果,可以省
时省力的测定二斑叶螨对阿维菌素的敏感性。
英文摘要:In order to find the best method for determining the sensitivity of Tetranychus urticae Koch to abamectin, the effectiveness
of 5 assays; the microtiter plate method, the glass tube residual film method, the centrifugal tube residual film method,
the slidedip method and the clingfilm covered petri dish method, was evaluated. The results show that the microtiter
plate and glass tube residual film method were not convenient, and that the centrifugal tube residual film method barely
determined whether T. urticae adult individuals were alive or dead. The slidedip method was very slow but the clingfilm
covered petri dish method proved a convenient and efficient way to detect the sensitivity of T. urticae to abamectin 3 hours
after treatment with this pesticide.