
东亚飞蝗脑的形态学观察及三维重建
Morphological observation and 3D reconstruction of the brain of Locusta migratoria manilans
连国云1**,李德智2,陈义昆1,娄延霞2,刘志刚1***
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作者单位:1.深圳大学医学院深圳518060;2.中国农业科学院植物保护研究所农业部生物防治重点开放实验室北京100081
中文关键词:东亚飞蝗, 脑部, 形态结构, 三维重
英文关键词:Locusta migratoria manilensis, brain, morphological structure, 3D reconstruction
中文摘要: 本文主要研究东亚飞蝗Locusta migratoria manilansis (Meyen)脑部的形态结构及其三维重建模型。采用石蜡包埋切片,在光镜下观察了东亚飞蝗脑部的形态结构,其由前脑、中脑和后脑3部分组成。为了获得整只蝗虫的连续、完整的图像数据集,采用冰冻切片技术将冰冻包埋剂( OCT) 包埋的飞蝗成虫做连续切片。然后利用图像处理方法对飞蝗脑部的连续切片进行配准、分割,再用三维重建软件ImagePro Plus(IPP)对分割后的脑部二维图像序列进行三维重建,构建出的飞蝗脑部三维结构模型可以任意旋转,能从不同角度观察。其结果为蝗虫生理和防蝗治蝗提供科学依据。
英文摘要:The morphological structure of the brain of Locusta migratoria manilansis (Meyen) was observed and a 3D digital model of the brain developed. The brain was dissected and observed under light microscopy. The brain was found to consist of a protocerebrum, deutocerebrum and tritocerebrum. In order to obtain a 2D sectional image dataset of the locust, male adults were embedded in frozen embedding medium (OCT) and sliced into sections, and a 2D image of each section was stored in a dataset. After registering and segmenting the brain regions in the serial section images using image processing methods, ImagePro Plus (IPP) was used to reconstruct a 3D model of the brain which can be freely rotated and observed from any angle. This model provides a basis for scientific research on grasshopper physiology and locust control.