二斑叶螨对螺螨酯的抗性选育及其解毒酶活性测定

Determination of selection for resistance to spirodiclofen and its effect on detoxification enzymes in Tetranychus urticae

李忠洲**,周玉书***,朴静子,田如海,高萍

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作者单位：沈阳农业大学植物保护学院沈阳110866

中文关键词：二斑叶螨，螺螨酯，抗药性，解毒酶活性，杀螨剂

英文关键词：Tetranychus urticae, spirodiclofen, resistance, activity of detoxifying enzymes, acaricides

中文摘要：用螺螨酯对二斑叶螨Tetranychus urticae Koch进行35代抗性选育，抗性指数达到834，并在选育过程中，对二斑叶螨解毒酶的活性进行测定。结果表明，随着选育代数的增加，抗性增强，细胞色素P450活性亦显著提高，而羧酸酯酶、谷胱甘肽转移酶活性较敏感品系差异不显著。说明二斑叶螨对螺螨酯抗药性的增强与其体内细胞色素P450活性的增强有关。

英文摘要：Selection of the twospotted spider mite (Tetranychus urtica Koch) for resistance to spirodiclofen and its effect on detoxification enzymes were investigated. After 35 generations of continuous selection, resistance to spirodiclofen was > 834 that of the original susceptible strain. Odemethlyase activity of the F35 generation was significantly higher than that of the F0 generation. There was, however, no obvious difference in glutathione Stransferase or carboxy lesterase activity between the resistant and susceptible strains. We conclude that the resistance of T. urticae to spirodiclofen is related to enhancement of Odemethlyase activity.

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