蚊虫连续石蜡切片制备及染色
Preparation and staining of serial paraffin slices of mosquitoes.
谢超;赵彤言;杨发青;董言德;陆宝麟
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DOI:10.7679/j.issn.2095-1353.2000.100
作者单位:军事医学科学院微生物流行病研究所,北京100071
中文关键词:白纹伊蚊;塑化石蜡;HE染色
英文关键词:Aedes albopictus;paraplast;HE staining
中文摘要:本研究利用塑化石蜡进行了吸血前后白纹伊蚊整个蚊虫的石蜡包埋、制片及 HE染色 ,获得了染色清晰 ,组织完整的连续石蜡切片 ,并讨论了有关蚊虫石蜡包埋及制片染色的一些注意事项。为进一步利用免疫组化、原位 PCR等技术研究虫媒病毒在蚊虫体内的分布规律提供了可能
英文摘要:Whole Aedes albopictus was embeded in paraplast before and after meal blood.Serial paraffin sections were made and HE staining was processed.Tissues of the whole mosquito exhibited clear and different staining.This technique is valuable in studying arb