粉纹夜蛾细胞中Bt Cry1Ac选择抗性的研究及离体品系与毒素结合的比较
Comparison of studies on Cry1Ac-binding in resistant and susceptible cells in Trichoplusia ni BTI-Tn-5B1.
刘凯于;蒋才富;洪华珠;彭建新;余泽华
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DOI：
作者单位：华中师范大学昆虫学研究所,武汉430079
中文关键词：BtCry1Ac毒素;粉纹夜蛾BTI-Tn-5B1细胞;毒素结合多肽;抗性机制
英文关键词：Bt Cry1Ac toxin; BTI-Tn-5B1 cell; binding peptide; resistance mechanism
中文摘要：采用BtCry1Ac活性毒素对粉纹夜蛾BTI-Tn-5B1细胞进行 56代筛选后获得了抗性比为 1280倍的抗性细胞。ELISA检测表明抗性细胞总蛋白和膜蛋白结合的Cry1Ac数量都少于敏感细胞。配体结合Western杂交实验显示 :抗性细胞和敏感细胞的膜蛋白与总蛋白都有 5条电泳迁移率相同的毒素结合
英文摘要：The selected-resistance ratio to Cry1Ac toxin in BTI-Tn-5B1 reached 1?280 after 56 generations. ELISA showed that the quantity of Cry1Ac toxin bound by resistant cells was reduced. Western bolt analysis revealed that there were five different molecular we