绿盲蝽雌虫的浸提物分析
Identification of extracted compounds of female Lygus lucorum
苏建伟** 陈展册 张广珠 戈 峰
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DOI：
作者单位：中国科学院动物研究所虫害鼠害综合治理国家重点实验室 北京 100101
中文关键词：绿盲蝽， 浸提物， 气质联用， 反-2-丁酸己烯酯
英文关键词：Lygus lucorum, extract compounds, GC-MS, (E)-2-hexenyl butyrate
中文摘要：
采用浸泡法和气质联（ＧＣ-MS）用对绿盲蝽Lygus lucorum Meyer-dür雌虫不同部位的浸提物进行鉴定。结果表明：确定10种成分为绿盲蝽雌虫的浸提物中的主要组分，包括醇类，酸类和酯类。相对含量较高的依次有反-2-丁酸己烯酯、反-2-己烯醇、丁酸、顺-3-己烯醇、丁酸己酯。绿盲蝽雌虫在完成性召唤活动后，丁酸己酯、反-2-丁酸己烯酯和反-2-己烯醇的含量都明显减少，其中反-2-丁酸己烯酯从召唤前的7 200 ng减少到召唤后的250 ng，表明在绿盲蝽雌虫性召唤活动中，有大量的反-2-丁酸己烯酯释放到体外。比较绿盲蝽雌虫的不同部位的浸提物的组分发现反-2-丁酸己烯酯主要存在于虫体胸部，占总量的89.75%，且反-2-己烯醇也主要存在于胸部。因此推测反-2-丁酸己烯酯可能是绿盲蝽雌虫的性信息素成分或组分之一，并主要由胸部内的腺体分泌。
英文摘要： Semiochemicals extracted from virgin females of the green leaf bug, Lygus lucorum Meyer-dür, were analyzed by GC-MS. The volatiles extracted comprised ten compounds identified as esters, alcohols and acids. Among the compounds identified, (E)-2-hexenyl butyrate (E)-2-henenol, butyric acid, hexyl butyrate, and (Z)-3-henenol were the major components. Three compounds, hexyl butyrate, (E)-2-hexenyl butyrate and (E)-2-henenol significantly decreased with increased female calling activity. The fact the amount of (E)-2-hexenyl butyrate decreased from 7 200 ng per insect to 250 ng indicated that a large amount of (E)-2-hexenyl butyrate was emitted into the ambient air by females. Comparing the content of (E)-2-hexenyl butyrate in extracts from different parts of females, 89.75% was found in the thorax together with (E)-2-henenol. We suggest that (E)-2-hexenyl butyrate is a component of the sex pheromone of female L. lucorum that is secreted by a gland, or glands, in the thorax.