Bt工程菌WG-001与化学杀虫剂复配最佳配方筛选及田间防效研究
Screening of optimum proportion and field effect of mixtures of Bt engineering strain WG001 and chemical insecticides against Plutella xylostella
张聪冲，邱浩，覃春华，游红，李建洪**
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作者单位：华中农业大学植物科学技术学院武汉430070
中文关键词：苏云金芽孢杆菌工程菌WG-001，茚虫威，小菜蛾，增效作用，田间防效
英文关键词：wordsBacillus thuringiensis engineering strain WG-001, indoxacarb, Plutella xylostella, synergetic action, field effect
中文摘要： 为了延缓小菜蛾Plutella xylostella (L.)抗药性的产生和发展，筛选Bt工程菌WG-001与化学杀虫剂的最佳复配配方，本研究采用浸叶法分别测定了Bt工程菌WG-001与茚虫威、丁醚脲和巴丹复配对小菜蛾的增效作用。结果表明，当Bt工程菌WG-001与茚虫威以有效成分之比为100.10∶0.72复配时，增效作用最强，共毒系数为178.05。而Bt工程菌WG-001分别与丁醚脲和巴丹复配表现相加或拮抗作用。田间试验结果表明，Bt工程菌WG-001与茚虫威以100.10∶0.72的有效成分之比进行复配后，药后7 d，500倍液对小菜蛾的平均防效为8488％，显著优于Bt工程菌WG-001和茚虫威单用的平均防效。
英文摘要： The relative effectiveness of mixtures of the Bt strain WG-001 with the pesticides indoxacarb, diafenthiuron and cartap for controlling the diamondback moth (DBM) Plutella xylostella (L.) was determined using the leafdipping method. The results indicate that a 100.10∶0.72 mixture of Bt strain WG-001 and indoxacarb was the most synergetic with a cotoxicity coefficient (CTC) of 178.05. Mixtures of Bt strain WG-001 with diafenthiuron or cartap had an additive or antagonistic action, respectively. Results of a field trial showed that the average control effect of a 500 times dilution of the Bt strain WG-001 and indoxacarb mixture was 84.88％ 7 days after treatment, significantly higher than using either Bt WG-001 or indoxacarb separately (P＜0.05).