柞蚕微孢子虫孢子分离纯化方法
20Study on the method of separation and purification in Nosema pernyi
姜义仁1，2，邓真华2，王伯阳2，段玉玺1**，秦利2**
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DOI：
作者单位：1.沈阳农业大学植物保护学院沈阳110866；2. 沈阳农业大学生物科技学院沈阳110866
中文关键词：柞蚕，柞蚕微孢子虫，分离纯化
英文关键词：Antheraea pernyi, Nosema pernyi, separation and purification
中文摘要： 柞蚕微粒子病是柞蚕Antheraea pernyi(GuérinMéneville)的主要胚胎传染性病害，病原为柞蚕微孢子虫Nosema pernyi(Wen et Ding)，其病原分离提纯技术研究对于柞蚕微粒子病的防治具有重要意义。本文利用差速离心和Percoll密度梯度离心法研究了柞蚕微孢子虫孢子的分离纯化方法，结果表明，采用不连续密度梯度分离纯化柞蚕微孢子虫孢子的效果比单一浓度的效果好，以浓度为25%、50%、75%、100%不连续梯度，15 000 r/min离心30 min分离纯化得到的柞蚕微孢子虫孢子纯净度高。
英文摘要： Nosema pernyi (Wen et Ding) is a lethal pathogen caused pebrine disease in Antheraea pernyi (GuérinMéneville). Pebrine disease, which is vertically transmitted, is one of the most harmful diseases of A.pernyi. Better information on this disease and its vector are required to prevent outbreaks. This paper compares two methods of separating and purifying the spores of N.pernyi; differential centrifugation and Percoll density gradient centrifugation. The results show that discontinuous density gradient centrifugation was superior to single density gradient centrifugation. The purity of spores centrifuged at 15 000 r/min for 30 min under a discontinuous density gradient was 25%, 50%, 75% and 100%.