蔗根土天牛高致病力绿僵菌菌株的生物学特性研究
Biological characteristics of a strain of Metarhizium that is highly pathogenic to Dorysthenes granulosus
于永浩** ,龙秀珍 , 曾宪儒, 韦德卫 曾 涛***
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作者单位：广西农业科学院植物保护研究所/广西作物病虫害生物学重点实验室 南宁 530007
中文关键词：金龟子绿僵菌，培养条件，菌落生长，产孢量
英文关键词： Metarhizium anisopliae, culture condition, colony growth, spores yields
中文摘要：
【目的】 明确对蔗根土天牛Dorysthenes granulosus具有良好杀虫活性的金龟子绿僵菌Metarhizium anisopliae JC002菌株生长和产孢所需要的最适营养条件和环境条件。【方法】 通过单因素试验，测试了不同培养基、碳源、氮源、温度、pH及紫外线照射不同时间对JC002菌株生长和产孢的影响。【结果】 JC002菌株培养基的最佳配方是葡萄糖30 g、蛋白胨15 g、马铃薯200 g、琼脂20 g、水1 000 mL；蔗糖、NaNO3是菌落生长及产孢的最适碳源和氮源；菌株培养的最适温度为25℃，培养基的最适pH值为7.0；紫外光对菌株的生长速率无显著影响，但对产孢量有较大的抑制作用，紫外光照射时间越长，产孢越少。【结论】 本研究为JC002菌株的大量培养及其孢子制剂的大量生产和有效利用奠定基础。
英文摘要： [Objectives] The purpose of this paper was to explore the nutritional and environmental conditions for required for the hyphal growth and sporulation of Metarhizium anisopliae JC002, a newly isolated fungal strain with high insecticidal activity to the larvae of Dorysthenes granulosus. [Methods] Effects of different factors, such as culture medium, carbon sources, nitrogen sources, temperature, pH and UV on hyphal growth and sporulation were tested by single factor tests. [Results] The results indicate that the optimum formula for growing the JC002 strain was 30 g glucose, 15 g peptone, 200 g potatoes, 20 g agar and 1 000 mL water. Sucrose and NaNO3 were the best carbon and nitrogen source. The most suitable culture temperature was 25℃ and the optimum pH-value was 7.0. UV had no significant impact on the growth rate of the fungus but seriously inhibited its sporulation. [Conclusion] This study provides scientific basis for both the large-scale culture of the JC002 strain, and also the mass production and effective utilization of conidia of this strain.