蓟马科部分种类mtDNA-COⅠ序列变异及系统发育分析
Phylogenetic analysis of some Thripidae species based on sequence variation in the mtDNA-COⅠsegment
沈登荣1, 2** ,宋文菲3, 袁盛勇1, 田学军1, 和绍禹4 , 张宏瑞2***
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作者单位：1. 红河学院生命科学与技术学院 云南蒙自 661100；2. 云南农业大学植物保护学院 昆明 650201；3. 云南省农业科学院蚕桑蜜蜂研究所 云南蒙自 661101；4. 云南农业大学东方蜜蜂研究所 昆明 650201
中文关键词：蓟马科，系统发育，mtDNA-COⅠ，亲缘关系
英文关键词：Thripidae, phylogeny, mtDNA-COⅠ, genetic relationship
中文摘要：
【目的】 蓟马科是一类重要的经济昆虫，目前一些种类的传统形态系统分类还存在争议，本研究采用分子标记对蓟马科的系统发育进行探讨。【方法】 对蓟马科9属27种蓟马的mtDNA-COⅠ基因的变异进行分析，采用邻接法、最大似然法和贝叶斯法构建系统发育树。【结果】 研究表明在比对的432 bp的序列中，有207个保守位点，222个变异位点，3个缺失位点，序列A+T含量为68.3%，具有A、T偏倚性；蓟马种间的序列分歧度变化在15.6~40.0之间。【结论】 系统进化树支持针蓟马亚科为蓟马科最为原始的类群；棍蓟马亚科与绢蓟马亚科、蓟马亚科形成姐妹群；绢蓟马亚科与蓟马亚科有较近的亲缘关系，支持Mound四亚科的分类系统。
英文摘要：
[Objectives] The Thripidae consists of a lot of economically important insect pests. At present, the traditional morphological classification system remains controversial with respect to some species. In this paper, the phylogeny of Thripidae was investigated using molecular markers. [Methods] Sequence variation in the mtDNA-COⅠ gene of 9 genera and 27 species in the Thripidae was analyzed. Phylogenetic trees were constructed with Neighbor-Joining, maximum likelihood and Bayesian methods. [Results] The results show that there were 207 conservative sites, 222 variable sites and 3 missing loci in the 432 bp sequence analysed. The percentage of A+T was 68.3%. Sequence divergence among species ranged from 15.6 to 40.0. [Conclusion] The phylogenetic tree supports the hypotheses that the Panchaetothripinae are a relatively primitive group, the Dendrothripinae, Sericothripinae and Thripinae are a sister group, and that the Sericothripinae and Thripinae have a close genetic relationship. This supports Mound’s four subfamilies classification system.