热激蛋白90与中红侧沟茧蜂的发育关系
The relationship between the heat shock protein 90 and the development of Microplitis mediator
张 敏1**， 李建成2 ， 刘小侠1，张青文1***，赵章武1
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DOI：
作者单位：1. 中国农业大学农学与生物技术学院 北京 100193； 2. 河北省农林科学院植物保护研究所 保定 071000
中文关键词： 中红侧沟茧蜂； Hsp90；滞育； RACE； 半定量RT-PCR
英文关键词：Microplitis mediator， Hsp90， diapause， RACE， semiquantitative RT-PCR
中文摘要：根据热激蛋白90(the heat shock proteins 90，Hsp90)基因序列的保守性，设计引物，采用PCR结合RACE扩增的方法克隆得到中红侧沟茧蜂Microplitis mediator Haliday Hsp90cDNA全序列，并用半定量RT-PCR的方法研究Hsp90与中红侧沟茧蜂发育及滞育间的关系。结果表明，滞育条件和非滞育条件下，热激和冷激都能诱导Hsp90的表达；不论在滞育还是非滞育条件下，Hsp90表达量均在2龄初期最高，随发育时间的增加逐渐降低，成虫期表达量又显著升高；滞育褐茧在4℃保存初期，Hsp90呈现高表达且随保存时间的增加而降低。
英文摘要：The heat shock protein 90 gene (hsp90) from Microplitis mediator Haliday was isolated through PCR, RACE and confirmed by semiquantitative PCR. The results revealed that both heat shock and cold shock could induce the expression of Hsp90, no matter the larvae were under the condition of diapause or not; The highest expression level appeared at the early stage of the 2ndinstar larvae, whereas the expression level decreased with development, the expression of Hsp90 increased after the adult eclosion. During the maintenance period under 4℃, the expression of Hsp90 showed significant increment at the initial days but the level declined with the elongation of maintenance time.