【目的】 昆虫求偶交配行为与性信息素合成释放存在协调一致性，通过对棉红铃虫Pectinophora gossypiella (Saunders)生殖行为的研究，可以为探索红铃虫性信息素合成释放规律及利用性信息素监测防治红铃虫奠定基础。【方法】 本试验在室内条件下，研究了日龄、交配史等因素对红铃虫雌蛾求偶、交配行为规律的影响。【结果】 红铃虫雌蛾的求偶、交配行为均发生在黑暗条件下。雌蛾求偶行为在黑暗处理5 h后开始，7-9 h达到最高峰。雌蛾交配行为开始于暗期6.5 h后，8-9 h达到高峰。雌蛾的求偶百分率、求偶次数、开始求偶时间、求偶高峰持续时间受成虫日龄影响显著。其中，处女蛾1-13日龄的求偶率最高，4-15日龄的求偶次数最多、求偶高峰持续时间最长，其开始求偶出现的时间随日龄增加有逐渐提前的现象。红铃虫雌蛾交配率在羽化后第1天最高，之后随日龄逐渐呈下降趋势。相比处女蛾，有一次交配经历和多次交配经历的雌蛾平均寿命相对较低，但处理间差异不显著。有交配经历雌蛾的求偶率及求偶持续时间基本都低于处女雌蛾。【结论】 红铃虫求偶、交配行为的变化规律与成虫日龄密切相关，两者间具有一定时间上的协调性。红铃虫雌蛾寿命与交配行为呈显著的负相关性（相关系数r=﹣0.350，P=0.000<0.01）。交配经历对红铃虫雌蛾后续的求偶率、求偶持续时间均有一定影响。

[Objectives]  To investigate the reproductive behavior of Pectinophora gassypiella in order to establish a basis for using sex pheromones to monitor and control this pest. [Methods]  The effects of age and mating history on the courtship and mating behavior of P. gossypiella females were studied in a laboratory. [Results]  The courtship and mating behavior of P. gossypiella could only be observed in the scotophase. Females commenced calling 5 h after dark, reaching a peak in the 7^th-9^th hour of the scotophase. Mating mostly occurred 6.5 h after dark, reaching a peak during the 8^th and the 9^th hour of the scotophase. Age was closely related to the percentage of time spent calling and mating, number of calling bouts, time of first calling, and calling duration. The percentage of time calling reached a maximum at 1-13 days of age. The mean number of calling bouts, and calling duration during the peak period, remained at a high level from day 4 to day 15 and the time of first calling advanced with age. The mating peak occurred on the day of emergence, after which the mating frequency of females decreased with age. Compared to virgin females, the average longevity of mated females and those that had mated multiple times was relatively low, but this difference was not significant. The courtship rate and courtship duration of mated females were lower than those of virgin females. [Conclusion]  The courtship and mating behavior of P. gossypiella was closely related to female age. There was a significant negative correlation between mating and longevity in females (r=－0.350，P=0.000<0.01). Mating also affected the percentage of time spent calling and calling duration.