【目的】 通过比较松毛虫赤眼蜂Trichogramma dendrolimi滞育预蛹（Diapause prepupa, Dre）与非滞育预蛹（Non-diapause prepupa，NDre）间的代谢差异，找出差异代谢物及差异代谢通路，为松毛虫赤眼蜂滞育的机制奠定理论基础。【方法】 采用液相色谱-质谱技术进行代谢组学分析，通过PCA（Principal components analysis）、PLS-DA（Partial least squares discriminant analysis）、OPLS-DA（Orthogonal partial least squares discriminant analysis）进行模型分析及检验，通过t检验P值、VIP值、差异倍数比较出两组间差异代谢物，通过KEGG富集分析筛选出差异代谢物的显著富集通路。【结果】 在松毛虫赤眼蜂滞育预蛹和非滞育预蛹中共筛选出1 072种差异代谢物，其中674种在滞育期间显著上升，398种显著下降，差异最显著的物质主要包括磷脂、溶血磷脂、氨基酸及其衍生物、碳水化合物等。KEGG通路富集分析显示多种氨基酸的合成、代谢及羧酸循环通路变化显著，说明松毛虫赤眼蜂在滞育期间能量物质发生了显著的含量变化和相互转化，从而提高了耐寒性。【结论】 初步探究了滞育松毛虫赤眼蜂与非滞育松毛虫赤眼蜂的代谢差异，为松毛虫赤眼蜂滞育期间的代谢调控机制及途径提供理论依据。

[Objectives]  To compare metabolic differences between diapausing (Dpre) and non-diapausing Trichogramma dendrolimi prepupae (NDpre) in order to better understand the diapause mechanism of this species. [Methods]  Liquid Chromatography-Mass Spectrometry (LC-MS) was used for metabonomic analysis. PCA, PLS-DA and OPLS-DA were used for model analysis and statistical tests. Differences in metabolites between treatment groups were compared using the P-value in t-tests, VIP-values and fold change. Significant enrichment pathways of differential metabolites were screened using KEGG enrichment analysis. [Results]  A total of 1 072 differential metabolites were identified. Among these, 674 significantly increased, and 398 significantly decreased, during diapause. These were mainly phospholipids, lysophosphatides, amino acids and their derivatives, and carbohydrates. KEGG pathway enrichment analysis indicated that there were significant changes in the synthesis and metabolism of various amino acids, and in the tricarboxylic acid cycle pathway, indicating significant changes in, and mutual transformation of, energy-storing substances that improve cold tolerance during diapause. [Conclusions]  A preliminarily investigation of metabolic differences between diapausing and non-diapausing T. dendrolimi prepupae sheds light on the metabolic regulation mechanisms and pathways regulating diapause in this species.