【目的】  探究九香虫Aspongopus chinensis水煎液对乳腺癌细胞MDA-MB-453和HCC1937增殖、迁移、周期和凋亡的影响。【方法】 采用细胞增殖-毒性检测试剂盒（Cell Counting Kit-8，CCK-8）检测九香虫水煎液对两种乳腺癌细胞体外增殖的影响，细胞划痕、流式细胞术检测乳腺癌细胞迁移能力、细胞周期分布和细胞凋亡的影响，并通过实时荧光定量PCR（RT-PCR）检测Caspase-3、Caspase-8、Bax和Bcl-2（细胞凋亡相关的基因），MMP-2和MMP-9（细胞迁移相关的基因）及CDK-1、CDK-2、CyclinA1和CyclinB1（细胞周期相关的基因）的表达变化。【结果】 九香虫水煎液可抑制乳腺癌细胞MDA-MB-453和HCC1937体外增殖，通过下调MMP-2和MMP-9抑制乳腺癌细胞的迁移能力，并通过上调Caspase-3、Caspase-8和Bax及下调Bcl-2表达，诱导乳腺癌细胞凋亡。九香虫水煎液可阻滞MDA-MB-453细胞于G1期，阻滞HCC1937细胞于G2期，作用机制可能是下调CDK-1、CyclinA1和CyclinB1表达。【结论】 九香虫水煎液可通过抑制细胞增殖和迁移、阻滞细胞周期和诱导细胞凋亡等多种途径抑制乳腺癌细胞生长。

[Aim]  To investigate the effects of a decoction of Aspongopus chinensis (ACD) on the proliferation, migration, cell cycle and apoptosis, of MDA-MB-453 and HCC1937 breast cancer cells. [Methods]  The CCK-8 method was conducted to detect the effect of ACD on the proliferation of breast cancer cells in vitro and a cell wound healing assay was used to investigate the effect of ACD on their migratory ability. The cell cycle distribution and apoptosis rate were detected using flow cytometry. The mRNA expression of Caspase-3, Caspase-8, Bax and Bcl-2 (Genes associated with apoptosis), MMP-2 and MMP-9 (Genes involved in cell migration), CDK-1, CDK-2, CyclinA1 and CyclinB1 (Cell cycle related genes) were detected using RT-PCR. [Results]  ACD inhibited the proliferation and migration of breast cancer cells in vitro by down-regulating MMP-2 and MMP-9. It also induced apoptosis by up-regulating Caspase-3, Caspase-8 and Bax and by down-regulating Bcl-2. It blocked MDA-MB-453 cells in the G1 phase, and HCC1937 cells in the G2 phase, possibly by down-regulating CDK-1, CyclinA1 and CyclinB1. [Conclusion]  ACD can inhibit the proliferation and migration of MDA-MB-453 and HCC1937 breast cancer cells, regulate the cell cycle of these cells and induce their apoptosis in vitro.