伏马毒素B1诱导棉铃虫HaCYP6AE19表达进而增强对高效氯氟氰菊酯的抗药性
Fumonisin B1 induces the expression of HaCYP6AE19 in Helicoverpa armigera, thereby enhanceing resistance to λ-cyhalothrin
郑小虎** 赵倩倩 庞民好 杨太新 刘颖超*** 唐博文***
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DOI：10.7679/j.issn.2095-1353.2024.121
作者单位：河北农业大学植物保护学院，保定 071000
中文关键词：棉铃虫；伏马毒素B1；HaCYP6AE19；高效氯氟氰菊酯；抗药性
英文关键词：Helicoverpa armigera; HaCYP6AE19; fumonisin B1; λ-cyhalothrin; insecticide resistance
中文摘要：
【目的】 玉米耕作模式的转变使得伏马毒素B1在玉米组织中积累，棉铃虫Helicoverpa
armigera在取食玉米时便会摄入伏马毒素B1，但这对棉铃虫的抗药性有何影响未见报道。近年来，棉铃虫对高效氯氟氰菊酯的抗药性逐年升高，这是否与棉铃虫取食伏马毒素B1有关尚不得知。本研究将以此为目的展开探讨，明确棉铃虫对高效氯氟氰菊酯抗药性升高的原因，为害虫抗药性产生提供新思路。【方法】 在棉铃虫饲料中添加伏马毒素B1，测定了伏马毒素B1对棉铃虫抗药性的影响，通过转录组测序和RNAi技术鉴定了关键解毒基因，利用RT-qPCR验证了伏马毒素B1和高效氯氟氰菊酯对解毒基因表达量的影响。【结果】 取食0.01和0.1 mg/kg伏马毒素B1后，高效氯氟氰菊酯对棉铃虫的LC50值分别为对照组的1.31和1.43倍；转录组测序鉴定到关键解毒基因HaCYP6AE19，在沉默HaCYP6AE19后，高效氯氟氰菊酯对棉铃虫的LC50值为对照组的2.59倍；在伏马毒素B1和高效氯氟氰菊酯胁迫下，1 d后，HaCYP6AE19表达量无明显变化（P>0.05），2 d后，表达量分别为对照组的4.44（P<0.01）和2.39倍（P<0.05），3 d后，表达量分别为对照组的5.27（P<0.01）和6.25倍（P<0.01）。【结论】 摄入伏马毒素B1后，棉铃虫解毒基因HaCYP6AE19过度表达，进而增强了棉铃虫对高效氯氟氰菊酯的抗药性。
英文摘要：
[Aim] To
investigate whether the ingestion of fumonisin B1 in maize tissues
by Helicoverpa armigera could contribute to the increase in resistance
of this pest to λ-cyhalothrin. [Methods] Fumonisin B1 was added to the
feed of H. armigera and its effect on the resistance of H. armigera to λ-cyhalothrin was determined. Key detoxification genes were identified by
transcriptome sequencing and RNAi technology, and the effects of fumonisin B1 and λ-cyhalothrin on the expression of these genes was verified with RT-qPCR. [Results]
After feeding on a diet containing
0.01 and 0.1 mg/kg of fumonisin B1, the LC50 values of H.
armigera to λ-cyhalothrin were 1.31 and 1.43 times higher, respectively,
than those of the control group. The key detoxification gene HaCYP6AE19 was identified by transcriptome sequencing. After silencing this gene, the LC50 value of H. armigera to λ-cyhalothrin was 2.59 times that of the control
group. There was no significant change in HaCYP6AE19 expression after 1
day (P>0.05) of either fumonisin B1 or λ-cyhalothrin
stress. However, after 2 days the expression of this gene in the fumonisin B1 and λ-cyhalothrin treatment groups was 4.44 (P<0.01) and 2.39 (P<0.05)
times, respectively, that of the control, and after 3 days, its expression was
5.27 (P<0.01) and 6.25 (P<0.01) times, respectively, that
of the control. [Conclusion] Ingestion
of fumonisin B1 by H. armigera causes overexpression
of the detoxification gene HaCYP6AE19 and thereby enhances resistance to
λ-cyhalothrin.