【目的】 探讨外源Wolbachia注入对于柑橘木虱Diaphorina citri营养与免疫应答的影响，以明确Wolbachia-营养代谢-免疫应答三者之间的互作关系，为今后研究利用Wolbachia防控柑橘木虱提供理论基础。【方法】 对柑橘木虱高龄若虫通过显微注射使其感染外源Wolbachia，使用全自动氨基酸分析仪对柑橘木虱体内氨基酸含量、种类变化进行检测；利用Diff-Quick染色法、酶活检测试剂盒检测血细胞含量、溶菌酶活性。【结果】 外源Wolbachia感染后，处理组柑橘木虱体内游离氨基酸总量显著高于对照组种群（P=0.018 4），其中必需氨基酸含量无显著差异，而非必需氨基酸含量显著高于对照组种群（P=0.039 1）；氨基酸种类比较结果显示，处理组种群和对照组种群中均检测到了31种游离氨基酸，在9种必需氨基酸中，苏氨酸（Thr）在处理组种群中的比例显著低于对照组种群（P=0.013 2），22种非必需氨基酸中，处理组种群α-氨基己二酸（α-AAA）（P=0.028 4）、胱硫醚（Cysthi）（P=0.012 6）、甘氨酸（Gly）（P=0.042 9）和氨（NH₃）（P=0.035 2）4种非必需氨基酸所占比例显著低于对照组种群。Wolbachia感染24 h后，处理组血淋巴内的血细胞数量（P=0.047 1）、溶菌酶活性（P=0.013 2）显著高于对照组，但48 h后处理组与对照组体内血细胞数量、溶菌酶活性均有所下降，两者差异不显著。【结论】 外源Wolbachia的注入对柑橘木虱的营养生理产生了显著影响，导致体内游离氨基酸含量增加，苏氨酸、α-氨基己二酸、胱硫醚、甘氨酸及氨的比例降低；相反柑橘木虱通过激活免疫反应来增加血细胞含量和溶菌酶活性以适应外源Wolbachia感染，维持正常的生命活动。

 [Aim]  1. To investigate the effect of exogenous Wolbachia injection on the nutritional and immune responses in Asian citrus psyllids (Diaphorina citri). 2. To clarify the relationship between Wolbachia, nutrient metabolism, and the immune response in D. citri. 3. To provide a theoretical basis for a sustainable Wolbachia-based control strategy for D. citri. [Methods]  D. citri were microinjected with exogenous Wolbachia, and the amino acid content and species changes were detected in vivo using an automatic amino acid analyzer. Hemocyte content and lysozyme activity were analyzed using Diff-Quick staining and enzyme activity assays. [Results]  The total amount of free amino acids in exogenous Wolbachia injected D. citri (EW) was significantly higher compared to the control population (P=0.018 4). There was no significant difference in the amount of essential amino acids between the EW and control populations. In contrast, the EW population had a significantly higher level of non-essential amino acids than the control population (P=0.039 1). Amino acid species comparison detected 31 free amino acids in both the EW and control populations. Of the 9 essential amino acids, the proportion of threonine (Thr) was significantly lower in the EW population than the control population (P=0.013 2). Of the 22 non-essential amino acids, α-amino adipic acid (α-AAA) (P=0.028 4), cystathionine (Cysthi) (P=0.012 6), glycine (Gly) (P=0.042 9), and ammonia (NH3) (P=0.035 2) levels were significantly lower in the EW population than the control population. The hemocyte quantities (P=0.047 1) and lysozyme activities (P=0.013 2) in the haemolymph of the EW population were significantly higher compared to the control population 24 h after Wolbachia infestation. There was a decline in hemocyte quantity and lysozyme activity in vivo in the EW and control populations after 48 h, with no significant difference between the two populations. [Conclusion]  Injecting exogenous Wolbachia significantly affected the trophophysiology of D. citri, resulting in an increase in free amino acid content and a decrease in threonine, α-aminoadipic acid, cystathionine, glycine, and ammonia. In contrast, hemocyte content and lysozyme activity were increased through activation of the immune response, indicating D. citri is able to adapt to exogenous Wolbachia infestation and maintain its regular activities.