【目的】 明确不同冷藏温度和时间对粗脊蚜茧蜂Aphidius colemani羽化率、成虫存活率、寿命及雌雄性比的影响，找出粗脊蚜茧蜂最适冷藏条件，为规模化繁育提供技术支撑。【方法】 以粗脊蚜茧蜂（僵蚜）为研究对象，在2、3、4、5和6 ℃温度下冷藏相同僵化程度的粗脊蚜茧蜂僵蚜5、10、15、20和25 d，记录羽化数、成活数、寿命及雌雄数量。【结果】 （1）冷藏温度、冷藏时间及其交互作用对僵蚜羽化率差异均不显著（P>0.05）；冷藏温度、冷藏时间对成虫存活率差异极显著（P<0.001），其交互作用对成虫存活率差异不显著（P>0.05）；冷藏温度及其和冷藏时间的交互作用对僵蚜羽化成蜂寿命差异不显著（P>0.05），对羽化成蜂雌雄性比差异极显著（P<0.001）；冷藏时间对羽化成蜂寿命差异显著（P<0.05）；对雌雄性比差异不显著（P>0.05）。（2）粗脊蚜茧蜂僵蚜羽化率处理与对照无显著差异（P>0.05），处理组羽化率在63.81%-89.52%之间。3 ℃冷藏15 d羽化率最高为89.52%，4、5和6 ℃冷藏10 d羽化率在80.00%以上，3和4 ℃冷藏25 d羽化率仍在82.86%以上。（3）低温冷藏过程中僵蚜均有羽化现象，成虫存活率在2-6 ℃整体随冷藏温度升高而逐步降低，在5-25 d随冷藏时间延长先升高后降低。冷藏处理10 d成虫存活率在43.83%-72.07%之间，显著高于其它处理（P<0.05）。（4）处理组与对照组羽化率无显著差异（P>0.05）。冷藏10 d成蜂寿命最长，显著高于冷藏5 d（P<0.05），5 ℃冷藏10 d寿命最长为4.37 d，2、3、4和5 ℃冷藏10 d羽化成蜂寿命在4.02 d以上，5 ℃冷藏15 d寿命仍在4.29 d以上。（5）僵蚜分别在2、4、5和6 ℃温度下，经过不同时间冷藏处理后羽化成蜂的雌雄性比与对照和3 ℃冷藏时差异显著（P<0.05）；2和4 ℃时在不同冷藏时间雌雄性比显著高于3和5 ℃（P<0.05）。【结论】 粗脊蚜茧蜂在低温冷藏过程均有羽化现象，根据羽化率、成虫存活率、寿命及雌雄性比，建议其在4或5 ℃下冷藏10 d保存。

[Aim]  The purpose of this study was to examine the effects of different cold storage temperatures and duration on the eclosion rate, survivorship, longevity, and sex ratio of Aphidius colemani, and determine the optimal storage conditions for large-scale breeding of A. colemani. [Methods]  The eclosion rate, survivorship, longevity, and sex ratio of A. colemani were measured under different temperatures (2, 3, 4, 5 and 6 ℃) using aphid mummies. Subsequently, each temperature treatment group was stored for 5, 10, 15, 20, and 25 d. [Results]  The findings indicated that there were no significant effects of cold storage temperature, cold storage duration, or the interaction between cold temperature and storage duration on the eclosion rate of aphid mummies (P>0.05). However, there was a significant effect of cold storage temperature and cold storage duration on aphid survivorship, but there was significant effect of the interaction between cold storage temperature and cold storage duration (P<0.001). Adult longevity was not significantly affected by either storage temperature or the interaction between cold storage temperature and cold storage duration (P>0.05). The adult sex ratio was significantly affected by both the storage temperature and the interaction between storage temperature and storage duration (P<0.001). Cold storage duration significantly affected the longevity of emerged adults (P<0.05), but not the sex ratio (P>0.05). There was no significant difference in the eclosion rate of A. colemani between the treatment group and the control group, with the eclosion rate ranging from 63.81% to 89.52 % for both groups. The highest eclosion rate was 89.52 % after 15 d of cold storage at 3 ℃. All eclosion rates were above 80.00% after 10 d of cold storage at 4, 5 and 6 ℃. The eclosion rate remained over 82.86% after 25 d of cold storage at 3 and 4 ℃. All A. colemani temperature treatment groups survived low temperature storage. Survivorship gradually decreased as the storage temperature increased from 2 to 6 ℃. Additionally, survivorship initially increased then decreased as the cold storage duration increased from 5 to 25 d. The survivorship ranged from 43.83% to 72.07% after 10 d of cold storage, representing the highest values observed across all the storage duration treatment groups (P<0.05). There was no significant difference in A. colemani longevity between the treatment and control groups (P>0.05). The longevity of the emerged wasps was highest after 10 d of cold storage and significantly higher than 5 d of cold storage (P<0.05). A. colemani stored at 5 ℃ for 10 d had the longest lifespan, reaching 4.37 d. When subjected to a 10 d refrigeration period, the longevity of the wasps exceeded 4.02 d for all temperature treatment groups. After 15 d of cold storage at 5 ℃, the wasps survived up to 4.29 d. Following cold storage durations of 5, 10, 15, 20, and 25 d at 2, 4, 5, and 6 ℃, there was a significant difference between the sex ratio of the wasps from the 3 ℃ treatment group and the control group compared to the other groups (P<0.05). The sex ratios collected from the 2 and 4 ℃ treatment groups were significantly higher compared to the 3 and 5 ℃ treatment groups (P<0.05). [Conclusion]  The findings of this research indicate A. colemani is still able to emerge during low temperature storage. A. colemani aphid mummies can be effectively stored between 4-5 ℃ for 10 d with minimal effect on the eclosion rate, survivorship, longevity, or sex ratio.