大螟不同地理种群的AFLP遗传分析
Genetic diversity of different geographical populations of Sesamia inferens Walker by AFLP
张志春等
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DOI：
作者单位：江苏省农业科学院植物保护研究所
中文关键词：大螟；地理种群；AFLP；遗传多态性
英文关键词：Sesamia inferens Walker; geographic population; AFLP; genetic diversity
中文摘要：为了明确我国大螟Sesamia inferens Walker不同地理种群的遗传多样性水平，利用AFLP方法对我国长江流域和南方稻区大螟的10个地理种群进行了遗传多样性分析，结果表明不同地理种群间具有丰富的遗传多样性，利用27对引物共扩增到1055条条带，其中837条条带具有遗传多态性，多态性比例为78.75%；种群间分化系数（Gst）为0.7132；Nei’s基因多样性（h）范围在0.2969～0.4547之间，平均为0.3752；Shannon信息指数介于0.2936～0.5043之间，平均为0.3888，；10个种群间遗传一致度较高，均大于0.63。遗传距离聚类分析表明，大螟10个地理种群可区分为4类，德阳，龙海，湛江各为一类，其余七个地理种群聚为一类。研究还表明，与其他测试种群的遗传距离普遍较大、相对孤立的地理种群，其聚类结果有一定的随机性，因此，取样点较多、且样点分布较均匀的聚类分析结果才有较高可信度。
英文摘要：To study the mechanism of the genetic diversity of different Sesamia inferens Walker populations, ten characteristic geographical populations from ten provinces of China were selected. With 37 pairs of AFLP selective primers, 1055 AFLP loci were recorded, of which 78.75% were polymorphic. Coefficient of gene differentiation (Gst) between populations was 0.7132, Nei’s gene diversity index (h) from 0.2969 to 0.4547 and the average was 0.3752, Shannon’s index from 0.2936 to 0.5043 and the average was 0.3888. There was high genetic identity between the 10 populations with the value greater than 0.63. NJ cluster analysis showed the ten populations are grouped into four major clades based on polymorphism of AFLP markers, of which DY, LH and ZJ populations belong to three different clades, and the other seven populations belong to one clade. The populations within each of the clades are consistently subject to similar geographic climate and near distance. However, the isolated population with longer genetic distance to all other tested populations was probably clustered into a random clade. So the sampled populations for genetic polymorphism clustering should be as many as possible and dispersed.