欧洲型舞毒蛾气味受体基因OrCo的克隆及序列分析
Molecular cloning and sequence analysis of the odorant receptor gene OrCo in Lymantria dispar (European gypsy moth)
秦 浩1, 2 ,于艳雪2**, 马 菲2, 张俊华2, 叶保华1, 陈乃中2
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DOI：
作者单位：1. 山东农业大学 泰安 271018；2. 中国检验检疫科学研究院植物检疫研究所 北京 100029
中文关键词： 欧洲型舞毒蛾，嗅觉受体，OrCo，基因克隆，序列分析
英文关键词： Lymantria dispra (European gypsy moth), olfactory receptor, sequence analysis
中文摘要：
【目的】 克隆欧洲型舞毒蛾Lymantria dispar Linnaeus的OrCo气味受体基因，并分析其序列特征，为进一步研究舞毒蛾嗅觉机制提供有益参考。【方法】 本研究利用RT-PCR和RACE方法，克隆获得欧洲型舞毒蛾 OrCo受体基因cDNA全长序列，将该基因命名为LdisOrCo, 在GenBank 中的登录号为KF482409。【结果】 序列分析结果显示，LdisOrCo开放阅读框全长为1 311 bp，编码436个氨基酸，序列中有7个跨膜区和高度保守的C端区域。序列联配分析表明，LdisOrCo基因的氨基酸序列与近缘种灰翅夜蛾（Spodoptera littoralis Or83b）的同源性高达89％，与鞘翅目台湾黑金龟（Holotrichia plumbea Or83b）同源性高达64％，与已经报道的其他昆虫的嗅觉受体同源性都在60％以上，特别是在C端几乎完全一致。【结论】 嗅觉受体OrCo（Olfactory receptor coreceptor）在不同昆虫体内高度保守，克隆欧洲型舞毒蛾OrCo基因可以为进一步研究舞毒蛾气味受体的功能，OrCo基因的进化以及揭秘嗅觉机制奠定基础。
英文摘要：
[Objectives] Exploring the mechanism of olfaction in Lymantria dispra (European gypsy moth) by cloning and analyzing the olfactory receptor coreceptor (OrCo) gene. [Methods] The full-length cDNA sequence of LdisOrCo was cloned using reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) (GenBank accession number KF482409). [Results] Sequence analysis revealed that the open reading frame (ORF) consisted of 1 311 nucleotides, encoding 436 amino acids with 7 putative transmembrane domains, and has a highly conserved C-terminus. Alignment analysis indicated that the nucleotide similarity of LdisOrCo with Or83b of Spodoptera littoralis and Holotrichia plumbea was 89%, 64%, respectively, and that it shares high homologies (>60%) with the corresponding receptor from other insects; the sequence of the C-terminus is almost the same. [Conclusion] The highly conserved olfactory receptor gene of Lymantria dispra (European gypsy moth) was sequenced in order to provide a basis for exploring the functions of the olfactory receptor, the evolution of the OrCo gene and the mechanism of insect olfaction.