刊期:双月刊
主管单位:中国科学院
主办单位:中国科学院动物研究所,中国昆虫学会
地址:北京市朝阳区北辰西路1号院5号中国科学院动物研究所
邮编:100101
电话:010-64807137
传真:010-64807137
E-Mail:entom@ioz.ac.cn
刊号:ISSN 2095-1353
        CN 11-6020/Q
国内发行代号:2-151
国际发行代号:BM-407
发行范围:国内外公开发布
定价:138元/册
定价:828元/年
银行汇款:中国工商银行北京海淀西区支行
户名:中国科学院动物研究所
帐号:0200 0045 0908 8125 063

您所在位置:首页->过刊浏览->2014年51卷第1期



苦瓜叶提取物对亚洲玉米螟的生物活性及对斜纹夜蛾卵巢细胞的毒力
Bioactivity of extracts of Momordica charantia leaves against Ostrinina furnacalis and their cytotoxicity toSpodoptera litura ovary cells
刘 欢** ,朱春亚 , 张茂新 , 凌 冰***
点击:1487次 下载:51次
DOI:
作者单位:(华南农业大学资源环境学院 昆虫生态研究室,广州 510642)
中文关键词:苦瓜叶提取物,亚洲玉米螟,斜纹夜蛾卵巢细胞,生物活性,细胞毒力
英文关键词: extracts of Momordica charantia leaves, Ostrinina furnacalis, Spodoptera litura ovary cell, bioactivity, cytotoxicity
中文摘要:  【目的】 为研究苦瓜Momordica charantia L.叶正丁醇萃取物对亚洲玉米螟Ostrinina furnacalis Güenée生长发育及生殖力的影响,并探讨其作用机理。【方法】 采用饲料混药法测定了苦瓜叶正丁醇萃取物对亚洲玉米螟幼虫存活率、发育历期和雌成虫产卵量的影响。【结果】 0.025%0.1%0.2%浓度的苦瓜叶正丁醇萃取物混合饲料饲喂亚洲玉米螟2龄幼虫3 d后,幼虫存活率明显降低,校正死亡率分别为27.43%39.23%54.93%,且发育历期明显延长,雌成虫产卵量与对照相比分别下降了12.63%22.74%37.27%。进一步的研究结果表明,苦瓜叶正丁醇萃取物对斜纹夜蛾卵巢细胞(SL)的形态、增殖和生长密度有明显的抑制作用。处理后的SL细胞皱缩变圆,细胞核偏移甚至消失,胞质外泄。处理244872 h后,对SL细胞增殖的IC50值分别为180.96158.54122.87 mg/mL;处理6~72 h后,SL细胞相对生长密度随药剂浓度和处理时间的增加而明显下降;用125 mg/mL浓度处理SL细胞2345 d后,SL细胞对葡萄糖吸收的抑制率分别为7.66%13.97%19.45%27.30%【结论】 苦瓜叶正丁醇萃取物对SL细胞表现出的较强毒杀作用可能是其导致亚洲玉米螟生殖能力降低的主要作用机制之一。  

 

 

 

英文摘要:

        [Objectives]  To ascertain the effect of n-butyl alcohol extracts of Momordica charantia leaves on the growing, development and fecundity of Ostrinina furnacalis (Güenée). [Methods]  The effects of n-butyl alcohol extracts of M. charantia leaves on the survival rate and the developmental duration of O. furnacalis larvae and the fecundity of O. furnacalis adult females were investigated by adding these extracts to the food provided to O. furnacalis larvae and adult females under laboratory conditions. [Results]  The results demonstrated that after treating the survival rate of second instar respectively. In addition, developmental duration of treated larvae was significantly longer than that of the control group, and the rates of fecundity inhibition were 12.63%, 22.74% and 37.27%, respectively. Extracts of M. charantia leaves had an apparent inhibitory effect on Spodoptera litura cell proliferation, cellular morphology and growth density; cells shrunk, cellular nuclei migrated and even disappeared and cytoplasm leaked out after treatment. The IC50 values were 180.96, 158.54 and 122.87 mg/mL at 24, 48 and 72 h after treatment, respectively. The growth density of cells decreased significantly compared with control cells, after 6-72 h of exposure with increasing concentration and the treatment time. Treating cells with 125 mg/mL n-butyl alcohol extracts of M. charantia leaves for 2, 3, 4 and 5 days inhibited glucose absorption by 7.66, 13.97, 19.45 and 27.30%, respectively. [Conclusion]  These results suggest that the n-butyl alcohol extracts of M. charantia leaves are strongly cytotoxic to Spodoptera litura cells, which may account for the observed decline in female fecundity of O. furnacalis.

读者评论

      读者ID: 密码:   
我要评论:
版权所有©2021应用昆虫学报》编辑部 京ICP备10006425号
本系统由北京菲斯特诺科技有限公司设计开发
您是本站第6332132名访问者