几种水稻对褐飞虱的抗性鉴定及抗性相关次生物质分析
Relative resistance of several rice varieties to Nilaparvata lugens (St?l) and analysis of resistance-related secondary substances
李明阳 姚宇波 徐 翔 贡常委 杨旭东 杨德斌 王学贵
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DOI：10.7679/j.issn.2095-1353.2020.142
作者单位：四川农业大学国家级作物科学试验教学示范中心，成都 611130；四川农业大学无公害农药研究实验室，成都 611130； 四川省农业农村厅植物保护站 成都 610041；邛崃市农业农村局，邛崃 611530； 崇州市农业技术推广服务中心植物保护与植物检疫站，崇州 611230
中文关键词：水稻；褐飞虱；抗虫性；次生物质；GC/MS
英文关键词：rice; Nilaparvata lugens; insect resistance; secondary substances; GC/MS
中文摘要：
【目的】 了解次生物质对褐飞虱的抗性机理，筛选高抗性的水稻种质资源，为水稻抗褐飞虱品种的选育及后续推广应用提供理论依据。【方法】 采用标准苗期集团筛选法（SSST法）评价了9份水稻种质资源对褐飞虱的抗性水平，测定了不同抗性水平材料叶片中黄酮、总酚和草酸等次生物质的含量，利用气相色谱/质谱（GC/MS）分析了高抗、中抗和敏感品种水稻幼苗叶片化学成分。【结果】 岳恢9113（Bph15）为高抗，抗性分数（AS）<60%；岳恢9113（Bph14）、R715、岳恢9113（Bph14+Bph15）、蜀恢527（Bph6）为中抗，抗性分数值介于61%-80%之间，TN1、F638、F669、F660表现为敏感, 抗性分数值>80%。岳恢9113中的黄酮含量为10.13 µg/mL，总酚含量为74.63 µg/mL，草酸含量为24.35 µg/mL，均高于敏感材料TN1。高抗水稻品种岳恢9113中的3-壬醇,1,2,6,7-二聚氧基-3,7-二甲基-乙酸盐、2,4-二甲基苯甲醛、2-甲氧基-4-乙烯基苯酚、2-氧戊二酸、2-正丙基苯酚含量高于中抗R715和敏感TN1两个水稻品种。【结论】 次生物质黄酮、总酚和草酸含量与水稻抗性呈显著正相关，3-壬醇,1,2,6,7-二聚氧基-3,7-二甲基-乙酸盐、2,4-二甲基苯甲醛、2-甲氧基-4-乙烯基苯酚这些物质可能与抗褐飞虱有一定相关性。
英文摘要：
[Objectives] To investigate the mechanism underlying the resistance of rice to Nilaparvata lugens (Stål), identify resistant rice varieties and provide a theoretical basis for the breeding and propagation of rice varieties resistant to N. lugens. [Methods] The resistance levels of nine rice varieties to N. lugens were evaluated with the Standard Seedbox Screening Technique. Secondary substances in rice leaves with different levels of resistance, including flavonoids, total phenols and oxalic acid, were detected and chemical constituents of rice leaves analyzed by gas chromatography/mass spectrometry (GC/MS). [Results] Yuehui 9113 (Bph15) was highly resistant with a resistance score (AS) of less than 60%, whereas Yuehui 9113 (Bph14), R715, Yuehui 9113 (Bph14 + Bph15) and Shuhui 527 (Bph6) were moderately resistant, with resistance scores ranging from 61% to 80%. TN1, F638, F669, F660 were susceptible to N. lugens, with resistance scores greater than 80%. The flavonoid and total phenol content of Yuehui 9113 were 10.13 g/mL and 74.63 g/mL, respectively, and the oxalic acid content (24.35 g/mL) was higher than the sensitive strain TN1 (15.93 g/mL). The contents of 3-Nonanol,1,2,6,7-diepoxy- 3,7-dimethyl-,acetate, Benzaldehyde, 2,4-dimethyl-, 2-Methoxy-4- vinylphenol, 2-Oxopentanedioic acid and Phenol, 2-propyl- in the high resistant strain of Yuehui 9113 were higher than in the two sensitive strains R715 and TN1. [Conclusion] The flavonoid, total phenol and oxalic acid content of rice varieties were positively correlated with resistance to N. lugens. In addition, 3-Nonanol, 1,2,6,7-diepoxy-3,7-dimethyl-, acetate, Benzaldehyde, 2,4-dimethyl- and 2-Methoxy-4-vinylphenol content may also be correlated with resistance to this pest.