棒尾小蛩螽线粒体基因组特征 及其系统进化研究
Mitochondrial genome characteristics and phylogeny of Microconema clavata
刘 菲，詹明德，王立志，束潇潇
点击：189次 下载：12次
DOI：10.7679/j.issn.2095-1353.2023.128
作者单位：陕西学前师范学院生命科学与食品工程学院
中文关键词：棒尾小蛩螽；线粒体基因组；蛩螽亚科；系统发育
英文关键词：Microconema clavata; mitochondrial genome; Meconematinae; phylogeny
中文摘要：
【目的】 棒尾小蛩螽隶属于直翅目螽斯科蛩螽亚科。目前蛩螽亚科的研究主要集中于形态分类，系统进化研究较少。为探究棒尾小蛩螽线粒体基因组特征，丰富蛩螽亚科线粒体基因组数据库，为蛩螽亚科线粒体基因组比较和系统发育研究提供数据支持，本研究对棒尾小蛩螽的线粒体基因组进行测定、组装、注释和分析，并探讨了棒尾小蛩螽系统发育地位和蛩螽亚科系统进化关系。【方法】 本研究采用高通量测序技术对棒尾小蛩螽基因组进行测序，组装获得完整的线粒体基因组序列，并进行注释和详细分析。选取包括棒尾小蛩螽在内的12个蛩螽亚科物种的线粒体基因组序列，利用13个蛋白编码基因和2个rRNA基因构建蛩螽亚科系统进化树。【结果】 棒尾小蛩螽线粒体基因组序列总长度为15 618 bp，包含13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和1个控制区，基因排列紧凑，具有昆虫祖先序列相同的基因排序；棒尾小蛩螽线粒体基因组A+T占总碱基的70.7%，存在明显的AT偏向性；13个蛋白编码基因中，除ND1以TTG为起始密码子外，其余均为ATN；除COX1、ND5和ND4使用不完整终止密码子T，其余均使用TAG或TAA作为终止信号；22个tRNAs中，除tRNASer(AGN)外均为典型的三叶草结构。【结论】 本研究获得了棒尾小蛩螽完整的线粒体基因组序列，贝叶斯系统树显示棒尾小蛩螽与格尼剑螽、斑翅简栖螽和斑腿栖螽的亲缘关系更近。
英文摘要：
[Objectives] To investigate the mitochondrial genome of Microconema clavata (Meconematinae, Tettigoniidae, Orthoptera), and thereby enhance the Meconematinae’s mitochondrial genome database to facilitate mitochondrial genome comparisons and phylogenetic research on this group. [Methods] We measured, assembled, annotated and analyzed, the mitochondrial genome of M. clavate, in order to determine the phylogenetic relationships of this species within the Meconematinae. We used next-generation sequencing technology to sequence the genome of M. clavate, assembled the complete mitochondrial genome sequence, then annotated and analyzed it in detail. The mitochondrial genome sequences of 12 species of the Meconematinae, including M. clavate, were selected to construct a phylogenetic tree based on differences in 13 protein coding genes and two rRNA genes. [Results] The total length of the mitochondrial genome sequence of M. clavate was 15 618 bp, which contained 13 protein coding genes, 22 tRNA genes, two rRNA genes and one control region. The genes were arranged compactly and had the same sequence as ancestral species. The A+T of the mitochondrial genome of M. clavate accounted for 70.7% of the total bases, and there was an obvious AT bias. With the exception of ND1 which began with TTG, the remainder of the 13 protein coding genes began with ATN. All genes have TAG or TAA as the termination signal, except COX1, ND5 and ND4, which have an incomplete termination codon T. All other tRNAs have a typical clover structure except tRNASer(AGN). [Conclusion] The complete mitochondrial genome sequence of M. clavate was obtained. A Bayesian phylogenetic tree indicates that M. clavata is most closely related to Xiphidiopsis gurneyi, Xizicus maculatus and Xizicus fascipes.