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Ywhae与Notch1在意大利蝗成虫不同组织和 不同发育阶段蝗卵的表达分析
Expression analysis of Ywhae and Notch1 in different adult tissues and eggs of Calliptamus italicus
蒋思涵,赵 娜,宋 余,季 荣,叶小芳
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DOI:10.7679/j.issn.2095-1353.2023.129
作者单位:新疆师范大学生命科学学院
中文关键词:意大利蝗;性别特征;Ywhae;Notch1
英文关键词:Calliptamus italicus; sex character; Ywhae; Notch1
中文摘要:

【目的】 YwhaeNotch1在调节昆虫胚胎发育和生育能力中具有重要作用。本研究旨在明确YwhaeNotch1是否为意大利蝗Calliptamus italicus的性别特征基因【方法】 基于意大利蝗雌、雄成虫的转录组数据,初步发现YwhaeNotch1为意大利蝗的雌雄差异表达基因,进一步采用qRT-PCR技术检测这2个基因在雌、雄成虫不同组织(体壁、肠道、卵巢和精巢)以及不同发育阶段蝗卵中的表达模式。【结果】 YwhaeNotch1在成虫各组织中均表达,雄虫所有组织中的Ywhae表达量均显著高于雌虫(P<0.05),Notch1表达量在雄虫体壁与精巢中极显著高于雌虫体壁和卵巢(P<0.01),雌虫肠道中的Notch1表达量则极显著高于雄性(P<0.001)。蝗卵发育过程中,早期发育阶段和滞育后发育阶段Ywhae表达量均极显著高于滞育阶段(P<0.01),且在滞育后发育阶段表达量升至最高(P<0.001);Notch1在早期发育阶段表达量最高(P<0.01),当蝗卵进入滞育时其表达量极显著下降(P<0.001),滞育后发育阶段其表达量极显著上升(P<0.01【结论】 YwhaeNotch1均为性别特征基因,且与蝗卵发育密切相关,在胚胎早期发育阶段可能参与诱导性别向雄性发育。研究结果可为深入揭示意大利蝗的性别分化机制提供线索。

英文摘要:

[Objectives]  To determine whether the Ywhae and Notch1 genes, which play important roles in the regulation of embryo development and fertility in insects, are differentially expressed in male and female Calliptamus italicus. [Methods]  Transcriptome data of adult male and females indicated that Ywhae and Notch1 are differentially expressed in the sexes of this species. We used qRT-PCR to detect the expression pattern of these two genes in different tissues (cuticle, intestine, ovary and testis) of female and male adults, and in eggs at different stages of development. [Results]  Both Ywhae and Notch1 were expressed in different adult tissues. Expression of Ywhae was significantly higher in male than in female tissues (P<0.05). Expression of Notch1 was significantly higher in the cuticle and testes of males than in cuticle and ovaries of females (P<0.01), and significantly higher in the intestines of females than males (P<0.001). Expression of Ywhae was significantly higher in eggs in the early-stage and in post-diapause stage, than in the diapause stage (P<0.01), and was highest in the post-diapause development stage (P<0.001). The expression of Notch1 was highest in eggs in the early development stage (P<0.01), and decreased significantly after eggs entered diapause (P<0.001). Notch1 expression increased significantly after the termination of diapause (P<0.01). [Conclusion]  Both Ywhae and Notch1 are differentially expressed in the sexes of C. italicus, and both genes are involved in the development of eggs. These genes are also involved in the development of the male reproductive system in the early embryonic stage. These results may provide clues revealing the mechanisms responsible for the differentiation of the sexes in C. italicus.

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